Adenovirus-mediated HSV-TK Gene Therapy Using hTERT Promoter in CNE Cells in vitro

被引：0
|
作者：
Zhang Yu [1 ,3 ]
Yu Xiang-hui [3 ]
Zha Xiao [1 ,2 ]
Kong Wei [3 ]
机构：
[1] Sichuan Tumor Hosp, Dept Radiotherapy, Chengdu 610041, Peoples R China
[2] Sichuan Tumor Hosp, Dept Mol Pharmacol, Chengdu 610041, Peoples R China
[3] Jilin Univ, Coll Life Sci, Vaccine Res Ctr, Changchun 130012, Peoples R China
来源：
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2009年 / 25卷 / 01期
基金：
中国国家自然科学基金;
关键词：
hTERT; HSV-TK; Adenovirus; Nasopharyngeal carcinoma; NASOPHARYNGEAL CARCINOMA;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Human telomerase reverse transcriptase(hTERT) activity was detected in human nasopharyngeal carcinoma cell(CNE) but not in human normal lung fibroblast(CCD-11Lu). Recombinant adenoviruses Ad-CMV-TK-enh and Ad-hTERT-TK-enh were constructed and infected into normal fibroblasts and nasopharyngeal carcinoma cells. Ad-CMv-TK-enh with 100 [mu mol/L of ganciclovir(GCV) caused 87% of CCD-11Lu cells death and 91% of CNE cells death, Ad-hTERT-TK-enh with 100 mu mol/L of GCV caused 24% of CCD-11Lu cells death and 79% of CNE cells death. These results indicate that the Ad-hTERT-TK-enh with GCV may be a useful method in suppressing tumor growth in targeted nasopharyngeal carcinoma gene therapy.
引用
收藏
页码：60 / 63
页数：4
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