Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial Dermatophagoides pteronyssinus Extracts

被引：147

作者：

Casset, Anne ^{[1
]}

Mari, Adriano ^{[4
]}

Purohit, Ashok ^{[5
]}

Resch, Yvonne ^{[1
]}

Weghofer, Margit ^{[1
]}

Ferrara, Rosetta ^{[4
]}

Thomas, Wayne R. ^{[6
]}

Alessandri, Claudia ^{[4
]}

Chen, Kuan-Wei ^{[1
]}

de Blay, Frederic ^{[5
]}

Valenta, Rudolf ^{[1
,2
]}

Vrtala, Susanne ^{[1
,3
]}

机构：

[1] Med Univ Vienna, Div Immunopathol, Dept Pathophysiol & Allergy Res, Ctr Pathophysiol Infectiol & Immunol, AT-1090 Vienna, Austria

[2] Med Univ Vienna, Christian Doppler Labs Allergy Res, AT-1090 Vienna, Austria

[3] Med Univ Vienna, Christian Doppler Labs Dev Allergen Chips, AT-1090 Vienna, Austria

[4] IDI IRCCS, Ctr Mol Allergol, Rome, Italy

[5] Strasbourg Univ Hosp, Div Asthma & Allergol, Dept Chest Dis, Strasbourg, France

[6] Univ Western Australia, Ctr Child Hlth Res, Telethon Inst Child Hlth Res, Perth, WA 6009, Australia

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY | 2012年 / 159卷 / 03期

基金：

奥地利科学基金会;

关键词：

House-dust mites; Allergen extracts; Dermatophagoides pteronyssinus; Skin prick tests; Recombinant allergens; HOUSE-DUST MITE; GRASS-POLLEN; BIOLOGICAL-ACTIVITY; BIRCH POLLEN; PRICK TEST; P; 7; RECOMBINANT; STANDARDIZATION; DER-P-1; DIAGNOSIS;

D O I：

10.1159/000337654

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity. Methods: Antibodies specific for several D. pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot. Results: Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0-40.8 mu g ml(-1); Der p 2: 1.7-45.0 mu g ml(-1)). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results. Conclusions: Commercially available D. pteronyssinus extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients. Copyright (C) 2012 S. Karger AG, Basel

引用

页码：253 / 262

页数：10

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