Immobilization of tyrosinase on chitosan-clay composite beads

被引：88

作者：

Dincer, Ayse ^{[1
]}

Becerik, Seda ^{[1
]}

Aydemir, Tulin ^{[1
]}

机构：

[1] Celal Bayar Univ, Fac Sci & Arts, Dept Chem, TR-45100 Muradiye, Manisa, Turkey

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 50卷 / 03期

关键词：

Tyrosinase; Immobilization; Chitosan; Clay; Composite beads; Phenol removal; POLYPHENOL OXIDASE; ENZYME IMMOBILIZATION; ACID-PHOSPHATASE; ALGINATE GEL; WASTE-WATER; REMOVAL; PHENOL; STABILIZATION; STABILITY; DEGRADATION;

D O I：

10.1016/j.ijbiomac.2011.11.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tyrosinase was immobilized on glutaraldehyde crosslinked chitosan-clay composite beads and used for phenol removal. Immobilization yield, loading efficiency and activity of tyrosinase immobilized beads were found as 67%, 25% and 1400 U/g beads respectively. Optimum pH of the free and immobilized enzyme was found as pH 7.0. Optimum temperature of the free and immobilized enzyme was determined as 25-30 degrees C and 25 degrees C respectively. The kinetic parameters of free and immobilized tyrosinase were calculated using L-catechol as a substrate and K-m value for free and immobilized tyrosinase were found as 0.93 mM and 1.7 mM respectively. After seven times of repeated tests, each over 150 min, the efficiency of phenol removal using same immobilized tyrosinase beads were decreased to 43%. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：815 / 820

页数：6

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