kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients

被引：7

作者：

Manoel-Caetano, Fernanda da Silva ^{[1
]}

Aparecida Carareto, Claudia Marcia ^{[1
]}

Borim, Aldenis Albaneze ^{[2
]}

Miyazaki, Kenji ^{[3
]}

Silva, Ana Elizabete ^{[1
]}

机构：

[1] Sao Paulo State Univ, UNESP, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil

[2] Sch Med, FAMERP, Dept Surg, Sao Jose Do Rio Preto, SP, Brazil

[3] Hosp Base, Serv Endoscopy, Sao Jose Do Rio Preto, SP, Brazil

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2008年 / 102卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

Chagas disease; Trypanosoma cruzi; Megaesophagus; kDNA minicircles; Genetic variability; LSSP-PCR;

D O I：

10.1016/j.trstmh.2008.05.027

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Trypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T cruzi kDNA minicircle gene signatures using the low-stringency single-specific-primer PCR technique in both peripheral blood and oesophageal. mucosa from chronic chagasic patients, with or without megaesophagus, atone or in combination with cardiopathy and megacolon. It was not possible to identify a uniform pattern of shared bands between blood and oesophageal mucosa samples from individuals with the same clinical. form or mixed forms, suggesting multiple T. cruzi infections with differential tissue tropism. Thus, the results indicate that there is an intense intraspecific variability in the hypervariable regions of T cruzi kDNA, which has so far made it impossible to correlate the genetic profile of this structure with the clinical manifestations of Chagas disease. (C) 2008 Royal. Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. AIL rights reserved.

引用

页码：1102 / 1107

页数：6

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