Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning

被引:65
作者
Van Tine, BA
Dao, LD
Wu, SY
Sonbuchner, TM
Lin, BY
Zou, NX
Chiang, CM
Broker, TR
Chow, LT
机构
[1] Univ Alabama Birmingham, Dept Biochem & Mol Genet, Birmingham, AL 35294 USA
[2] Univ Alabama Birmingham, Dept Mol Pathol, Birmingham, AL 35294 USA
[3] Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA
关键词
D O I
10.1073/pnas.0306848101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human papillomaviruses (HPVs) establish long-term infections in patients. The mechanism for extrachromosomal HPV DNA persistence in cycling cells is unknown. We show that HPV origin-containing plasmids partition as minichromosomes, attributable to an association of the viral origin recognition protein E2 with mitotic spindles. alpha-, beta-, and gamma-tubulins were pulled down with a tagged E2. The N-terminal transacting and C-terminal protein dimerization/DNA binding domains independently associated with the spindles. We suggest that this E2 property enables these viruses to establish persistence. Its implication for HPV oncogenesis is presented.
引用
收藏
页码:4030 / 4035
页数:6
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