Exosomes derived from HEK293T cells interact in an efficient and noninvasive manner with mammalian spermin vitro

被引：35

作者：

Vilanova-Perez, Teresa ^{[1
]}

Jones, Celine ^{[1
]}

Balint, Stefan ^{[2
]}

Dragovic, Rebecca ^{[1
]}

Dustin, Michael L. ^{[2
]}

Yeste, Marc ^{[3
]}

Coward, Kevin ^{[1
]}

机构：

[1] Univ Oxford, Nuffield Dept Womens & Reprod Hlth, John Radcliffe Hosp, Womens Ctr, Level 3, Oxford OX3 9DU, England

[2] Univ Oxford, Nuffield Dept Orthopaed, Kennedy Inst Rheumatol Rheumatol & Musculoskeleta, Roosevelt Dr, Oxford OX3 7FY, England

[3] Univ Girona, Inst Food & Agr Technol, Unit Cell Biol, Dept Biol Biotechnol Anim & Human Reprod TechnoSp, E-17003 Girona, Spain

来源：

NANOMEDICINE | 2020年 / 15卷 / 20期

基金：

英国惠康基金;

关键词：

delivery tool; exosome; infertility; reproductive medicine; sperm; EXTRACELLULAR VESICLES; IN-VITRO; MEDIATED DELIVERY; MEMBRANE; PROSTASOMES; CAPACITATION; FUSION; SIRNA; INFERTILITY; MICROVESICLES;

D O I：

10.2217/nnm-2020-0056

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim:To investigate exosomes as a noninvasive delivery tool for mammalian sperm.Materials & Methods:Exosomes were isolated from HEK293T cells and co-incubated with boar spermin vitro.Results: Internalised exosomes were detected within 10 min of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-h of exposure to exosomes, there were no significant deleterious effects with regard to sperm motility, viability, membrane integrity and mitochondrial membrane potential (p > 0.05), thus indicating that exosomes did not interfere with basic sperm function.Conclusion:HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertility.

引用

页码：1965 / 1980

页数：16

共 69 条

[1]

Aalberts M., 2013, REPRODUCTION, V147, P1

[2] Major protein alterations in spermatozoa from infertile men with unilateral varicocele [J].

Agarwal, Ashok ;

Sharma, Rakesh ;

Durairajanayagam, Damayanthi ;

Ayaz, Ahmet ;

Cui, Zhihong ;

Willard, Belinda ;

Gopalan, Banu ;

Sabanegh, Edmund .

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2015, 13

[3] Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes [J].

Alvarez-Erviti, Lydia ;

Seow, Yiqi ;

Yin, HaiFang ;

Betts, Corinne ;

Lakhal, Samira ;

Wood, Matthew J. A. .

NATURE BIOTECHNOLOGY, 2011, 29 (04) :341-U179

[4]

Amdani Siti Nornadhirah, 2016, Advances in Biological Regulation, V61, P58, DOI 10.1016/j.jbior.2015.11.009

[5] Nitric oxide and fusion with prostasomes increase cytosolic calcium in progesterone-stimulated sperm [J].

Arienti, G ;

Carlini, E ;

Saccardi, C ;

Palmerini, CA .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2002, 402 (02) :255-258

[6] Fusion of human sperm to prostasomes at acidic pH [J].

Arienti, G ;

Carlini, E ;

Palmerini, CA .

JOURNAL OF MEMBRANE BIOLOGY, 1997, 155 (01) :89-94

[7] Extracellular vesicle-mediated delivery of molecular compounds into gametes and embryos: learning from nature [J].

Barkalina, Natalia ;

Jones, Celine ;

Wood, Matthew J. A. ;

Coward, Kevin .

HUMAN REPRODUCTION UPDATE, 2015, 21 (05) :627-639

[8]

Barkalina N, 2015, NANOMEDICINE-UK, V10, P1539, DOI [10.2217/NNM.14.235, 10.2217/nnm.14.235]

[9] Using exosomes, naturally-equipped nanocarriers, for drug delivery [J].

Batrakova, Elena V. ;

Kim, Myung Soo .

JOURNAL OF CONTROLLED RELEASE, 2015, 219 :396-405

[10] Prostasomes as Potential Modulators of Tyrosine Phosphorylation in Human Spermatozoa [J].

Bechoua, Shaliha ;

Rieu, Isabelle ;

Sion, Benoit ;

Grizard, Genevieve .

SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE, 2011, 57 (03) :139-148

← 1 2 3 4 5 6 7 →