Long non-coding RNA XIST promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-367/141-ZEB2 axis in non-small-cell lung cancer

被引：159

作者：

Li, Chang ^{[1
,2
,3
]}

Wan, Liang ^{[1
,2
]}

Liu, Zeyi ^{[4
]}

Xu, Guangquan ^{[5
]}

Wang, Shengjie ^{[1
,2
,6
]}

Su, Zhiyue ^{[1
,2
]}

Zhang, Yingxi ^{[1
,2
]}

Zhang, Cuijuan ^{[1
,2
]}

Liu, Xia ^{[1
,2
]}

Lei, Zhe ^{[1
,2
,7
]}

Zhang, Hong-Tao ^{[1
,2
,7
]}

机构：

[1] Soochow Univ, Coll Med, Lab Canc Mol Genet, 199 Renai Rd,Sino Singapore Ind Pk, Suzhou 215123, Jiangsu, Peoples R China

[2] Soochow Univ, Coll Med, Sch Biol & Basic Med Sci, Dept Genet, Suzhou 215123, Jiangsu, Peoples R China

[3] Soochow Univ, Coll Med, Affiliated Hosp 1, Dept Cardiothorac Surg, Suzhou 215006, Jiangsu, Peoples R China

[4] Soochow Univ, Coll Med, Affiliated Hosp 1, Dept Resp Med, Suzhou 215006, Jiangsu, Peoples R China

[5] Harbin Med Univ, Affiliated Hosp 2, Dept Thorac Surg, Harbin 150086, Heilongjiang, Peoples R China

[6] Nanjing Med Univ, Kangda Coll, Dept Basic Med, Lianyungang 222000, Peoples R China

[7] Suzhou Key Lab Mol Canc Genet, Suzhou 215123, Jiangsu, Peoples R China

来源：

CANCER LETTERS | 2018年 / 418卷

基金：

中国国家自然科学基金;

关键词：

lncRNA XIST; Non-small-cell lung cancer (NSCLC); miR-141; Transforming growth factor beta (TGF-beta); EMT; DOWN-REGULATION; METASTASIS; INVASION; GROWTH; REPRESSION; CADHERIN; PROLIFERATION; PROGRESSION; STATISTICS; MECHANISMS;

D O I：

10.1016/j.canlet.2018.01.036

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Growing evidence shows that IncRNA XIST functions as an oncogene accelerating tumor progression. Transforming growth factor beta (TGF-beta)-induced epithelial-mesenchymal transition (EMT) plays a key role in tumor metastasis. However, it is still unclear whether IncRNA XIST is implicated in TGF-beta-induced EMT and influences cell invasion and metastasis in non-small-cell lung cancer (NSCLC). Here, we observed increased expression of IncRNA XIST and ZEB2 mRNA in metastatic NSCLC tissues. Knockdown of IncRNA XIST inhibited ZEB2 expression, and repressed TGF-beta-induced EMT and NSCLC cell migration and invasion. Being in consistent with the in vitro findings, the in vivo experiment of metastasis showed that knockdown of IncRNA XIST inhibited pulmonary metastasis of NSCLC cells in mice. In addition, knockdown of ZEB2 expression can inhibit TGF-beta-induced EMT and NSCLC cell migration and invasion. Mechanistically, IncRNA XIST and ZEB2 were targets of miR-367 and miR-141. Furthermore, both miR-367 and miR-141 expression can be upregulated by knockdown of IncRNA XIST. Taken together, our study reveals that IncRNA XIST can promote TGF-beta-induced EMT and cell invasion and metastasis by regulating miR-367/miR-141-ZEB2 axis in NSCLC. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：185 / 195

页数：11

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