Occult Hepatitis B Virus Infection in Chacma Baboons, South Africa

被引：10
作者：
Dickens, Caroline [1 ]
Kew, Michael C. [2 ,3 ]
Purcell, Robert H. [4 ]
Kramvis, Anna [2 ]
机构：
[1] Univ Witwatersrand, Dept Internal Med, ZA-2193 Johannesburg, South Africa
[2] Univ Witwatersrand, ZA-2193 Johannesburg, South Africa
[3] Groote Schuur Hosp, ZA-7925 Cape Town, South Africa
[4] NIH, Bethesda, MD 20892 USA
来源：
EMERGING INFECTIOUS DISEASES | 2013年 / 19卷 / 04期
基金：
美国国家卫生研究院; 新加坡国家研究基金会;
关键词：
MOLECULAR EPIDEMIOLOGY; NONHUMAN-PRIMATES; GENOTYPE-A; CHIMPANZEES; DNA; RELATEDNESS; SUBGENOTYPE; VARIANTS; SUBTYPE; DONORS;
D O I：
10.3201/eid1904.121107
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
During previous studies of susceptibility to hepatitis B virus (HBV) infection, HBV DNA was detected in 2/6 wild-caught baboons. In the present study, HBV DNA was amplified from 15/69 wild-caught baboons. All animals were negative for HBV surface antigen and antibody against HBV core antigen. Liver tissue from 1 baboon was immunohistochemically negative for HBV surface antigen but positive for HBV core antigen. The complete HBV genome of an isolate from this liver clustered with subgenotype A2. Reverse transcription PCR of liver RNA amplified virus precore and surface protein genes, indicating replication of virus in baboon liver tissue. Four experimentally naive baboons were injected with serum from HBV DNA positive baboons. These 4 baboons showed transient seroconversion, and HBV DNA was amplified from serum at various times after infection. The presence of HBV DNA at relatively low levels and in the absence of serologic markers in the baboon, a nonhuman primate, indicates an occult infection.
引用
收藏
页码：598 / 605
页数：8
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