Nonequilibrium fluctuations of a remodeling in vitro cytoskeleton

被引：73

作者：

Stuhrmann, Bjorn ^{[1
]}

Silva, Marina Soares E. ^{[1
]}

Depken, Martin ^{[2
]}

MacKintosh, Frederick C. ^{[2
]}

Koenderink, Gijsje H. ^{[1
]}

机构：

[1] FOM Inst Atom & Mol Phys AMOLF, Biol Soft Matter Grp, NL-1098 XG Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands

来源：

PHYSICAL REVIEW E | 2012年 / 86卷 / 02期

关键词：

MYOSIN; MECHANICS; DYNAMICS;

D O I：

10.1103/PhysRevE.86.020901

中图分类号：

O35 [流体力学]; O53 [等离子体物理学];

学科分类号：

070204 ; 080103 ; 080704 ;

摘要：

Motor proteins actively contract the actin cytoskeleton of cells and thereby give rise to nonequilibrium fluctuations as well as changes in the architecture of the cytoskeleton. Here, we show, by video microrheology of a reconstituted cytoskeleton, that motors generate time-dependent nonequilibrium fluctuations, which evolve as the network is remodeled. At earlier times, the fluctuation spectrum is dominated by strong non-Gaussian fluctuations, which arise from large displacements. At later times, directed displacements are infrequent and finally disappear. We show that these effects are due to contractile coarsening of the network into large actin-myosin foci.

引用

页数：5

共 29 条

[1] Temporal Analysis of Active and Passive Transport in Living Cells [J].

Arcizet, Delphine ;

Meier, Boern ;

Sackmann, Erich ;

Raedler, Joachim O. ;

Heinrich, Doris .

PHYSICAL REVIEW LETTERS, 2008, 101 (24)

[2] Active gels: dynamics of patterning and self-organization [J].

Backouche, F. ;

Haviv, L. ;

Groswasser, D. ;

Bernheim-Groswasser, A. .

PHYSICAL BIOLOGY, 2006, 3 (04) :264-273

[3] A quantitative analysis of contractility in active cytoskeletal protein networks [J].

Bendix, Paul M. ;

Koenderink, Glisje H. ;

Cuvelier, Damien ;

Dogic, Zvonimir ;

Koeleman, Bernard N. ;

Brieher, William M. ;

Field, Christine M. ;

Mahadevan, L. ;

Weitz, David A. .

BIOPHYSICAL JOURNAL, 2008, 94 (08) :3126-3136

[4] Nonequilibrium microtubule fluctuations in a model cytoskeleton [J].

Brangwynne, Clifford P. ;

Koenderink, Gijsje H. ;

MacKintosh, Frederick C. ;

Weitz, David A. .

PHYSICAL REVIEW LETTERS, 2008, 100 (11)

[5] Cytoplasmic diffusion: molecular motors mix it up [J].

Brangwynne, Clifford P. ;

Koenderink, Gijsje H. ;

MacKintosh, Frederick C. ;

Weitz, David A. .

JOURNAL OF CELL BIOLOGY, 2008, 183 (04) :583-587

[6] Cytoskeletal remodelling and slow dynamics in the living cell [J].

Bursac, P ;

Lenormand, G ;

Fabry, B ;

Oliver, M ;

Weitz, DA ;

Viasnoff, V ;

Butler, JP ;

Fredberg, JJ .

NATURE MATERIALS, 2005, 4 (07) :557-561

[7] Methods of digital video microscopy for colloidal studies [J].

Crocker, JC ;

Grier, DG .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 179 (01) :298-310

[8] Experimental evidence of strong anomalous diffusion in living cells [J].

Gal, Naama ;

Weihs, Daphne .

PHYSICAL REVIEW E, 2010, 81 (02)

[9] Hierarchical self-organization of cytoskeletal active networks [J].

Gordon, Daniel ;

Bernheim-Groswasser, Anne ;

Keasar, Chen ;

Farago, Oded .

PHYSICAL BIOLOGY, 2012, 9 (02)

[10] Nonlocal fluctuation correlations in active gels [J].

Head, D. A. ;

Mizuno, D. .

PHYSICAL REVIEW E, 2010, 81 (04)

← 1 2 3 →