Estimation of cardiomyocyte transmembrane potential with potential-sensitive fluorescent probes

被引：1
|
作者：
Khankoeva, AI
Dukhanin, AS
Galenko-Yaroshevskii, PA
机构：
[1] Kuban Med Acad, Dept Pharmacol, Krasnodar, Russia
[2] Russian State Med Univ, Dept Mol Pharmacol Radiobiol Course, Moscow 117437, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 1998年 / 126卷 / 11期
关键词：
transmembrane potential; antiarrhythmic drugs; cardiomyocytes;
D O I：
10.1007/BF02447265
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Transmembrane potentials on the plasma (Delta phi(p)) and mitochondrial (Delta phi(m)) membrane of isolated rat cardiomyocytes were estimated using the potential-sensitive fluorescent probe DSM. The values were -93+/-4 and -196+/-11 mV, respectively. Sufan significantly decreased the reduction of Delta phi(m) induced by chemical hypoxia. The effects of antiarrhythmic drugs on changes in Delta phi(p) were studied using the fluorescent probe dis-C-3-(5). Lidocaine, novocainamide, richlocaine, and leocaine blocked depolarization of the myocyte plasma membrane induced by electrical stimulation and did not affect the Delta phi(m).
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页码：1170 / 1172
页数：3
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