Combined enzymatic modification of stevioside and rebaudioside A

被引:58
作者
Kochikyan, VT [1 ]
Markosyan, AA
Abelyan, LA
Balayan, AM
Abelyan, VA
机构
[1] Natl Acad Sci Armenia, Inst Microbiol, Abovyan 375510, Armenia
[2] Biotechnol Corp Stevian, Kuala Lumpur 50450, Malaysia
关键词
D O I
10.1134/S0003683806010030
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by Bacillus stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. This method can be used successfully for direct transglycosylation of stevia extract without purification of its individual components.
引用
收藏
页码:31 / 37
页数:7
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