Spy vs. spy: selecting the best reporter for 19F NMR competition experiments

被引：22
|
作者：
de Castro, Guilherme Vieira [1 ]
Ciulli, Alessio [1 ]
机构：
[1] Univ Dundee, Sch Life Sci, Div Biol Chem & Drug Discovery, Dow St, Dundee DD1 5EH, Scotland
来源：
CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 10期
基金：
欧洲研究理事会; 英国惠康基金;
关键词：
E3 UBIQUITIN LIGASE; PROTEIN-PROTEIN INTERACTION; STRUCTURE-GUIDED DESIGN; SMALL MOLECULES; DRUG DISCOVERY; LEAD DISCOVERY; AFFINITY; OPTIMIZATION; LIBRARY; BINDING;
D O I：
10.1039/c8cc09790a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Systematic characterization of a series of fluorinated VHL ligands, varying binding affinity and position of the trifluoromethyl group, qualifies a spy molecule for competitive F-19 NMR screening and reveals guiding principles to develop highly sensitive assays with low material consumption.
引用
收藏
页码：1482 / 1485
页数：4
相关论文
共 23 条
  • [1] Chiral 19F NMR Reporter of Foldamer Conformation in Bilayers
    Wang, Siyuan
    della Sala, Flavio
    Cliff, Matthew J.
    Whitehead, George F. S.
    Vitorica-Yrezabal, Inigo J.
    Webb, Simon J.
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2022, 144 (47) : 21648 - 21657
  • [2] Rapid acquisition of 1H and 19F NMR experiments for direct and competition ligand-based screening
    Dalvit, Claudio
    Gossert, Alvar D.
    Coutant, Jerome
    Piotto, Martial
    MAGNETIC RESONANCE IN CHEMISTRY, 2011, 49 (04) : 199 - 202
  • [3] 29Si and 19F MAS NMR spectra of isolated 29Si(19F)2 and 29Si(19F)3 spin systems:: experiments and simulations
    Helluy, X
    Pietschnig, R
    Sebald, A
    SOLID STATE NUCLEAR MAGNETIC RESONANCE, 2003, 24 (04) : 286 - 300
  • [4] 19F multiple region selected 2D NMR correlation experiments
    Cheatham, S
    Groce, J
    JOURNAL OF FLUORINE CHEMISTRY, 2004, 125 (07) : 1111 - 1117
  • [5] 19F NMR reporter ligands in anion-metathesis equilibrium studies of Rh(I) systems.
    Hoffman, NW
    Cioffi, EA
    Huynh, HT
    Ledford, ND
    Laurilla, M
    Miller, SA
    Morris, CB
    Ream, JR
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2001, 221 : U675 - U676
  • [6] Application of BURBOP universal rotation pulses to 19F 2D NMR experiments
    Marchione, Alexander
    Diaz, Elizabeth
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255
  • [7] 19F NMR transverse and longitudinal relaxation filter experiments for screening: a theoretical and experimental analysis
    Dalvit, Claudio
    Piotto, Martial
    MAGNETIC RESONANCE IN CHEMISTRY, 2017, 55 (02) : 106 - 114
  • [8] Ligand-Based Competition Binding by Real-Time 19F NMR in Human Cells
    Luchinat, Enrico
    Barbieri, Letizia
    Davis, Ben
    Brough, Paul A.
    Pennestri, Matteo
    Banci, Lucia
    JOURNAL OF MEDICINAL CHEMISTRY, 2024, 67 (02) : 1115 - 1126
  • [9] Primary Amine Discrimination Using a 19F NMR Reporter Bearing two Different Fluorine-Containing Moieties
    Hamaguchi, Naoto
    Nishizawa, Rina
    Ikeda, Mayu
    Yokoyama, Miki
    Suzuki, Hiyono
    Matsumoto, Kouichi
    Ohta, Tetsuo
    Oe, Yohei
    CHEMISTRYSELECT, 2024, 9 (02):
  • [10] Simplification of 19F NMR spectra of liquid crystalline samples by multiple-pulse COSY experiments
    Lesot, P
    Emsley, JW
    Ouvrard, JM
    Curzon, E
    JOURNAL OF MAGNETIC RESONANCE, 1998, 133 (01) : 166 - 172
123