ZmMKK3, a novel maize group B mitogen-activated protein kinase kinase gene, mediates osmotic stress and ABA signal responses

被引：48

作者：

Zhang, Maoying ^{[1
]}

Pan, Jiaowen ^{[1
]}

Kong, Xiangpei ^{[1
]}

Zhou, Yan ^{[1
]}

Liu, Yang ^{[1
]}

Sun, Liping ^{[1
,2
]}

Li, Dequan ^{[1
]}

机构：

[1] Shandong Agr Univ, State Key Lab Crop Biol, Coll Life Sci, Shandong Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

[2] Taishan Med Univ, Fac Chem & Chem Engn, Tai An 271018, Shandong, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2012年 / 169卷 / 15期

关键词：

Abscisic acid; Hydrogen peroxide; Mitogen-activated protein kinase kinase; Osmotic stress; Transgenic tobacco; INDUCED ANTIOXIDANT DEFENSE; MAP KINASE; ABSCISIC-ACID; ZEA-MAYS; TRANSGENIC TOBACCO; OXIDATIVE STRESS; ARABIDOPSIS; PLANTS; TOLERANCE; CASCADE;

D O I：

10.1016/j.jplph.2012.06.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling modules and function as a convergent point for crosstalk during abiotic stress signaling. In this article, we isolated a novel group B MAPKK gene, ZmMKK3, from Zea mays. ZmMKK3 protein might be localized in both the cytoplasm and the nucleus. RNA blot analysis indicated that the ZmMKK3 transcription was up-regulated by abscisic acid (ABA), hydrogen peroxide (H2O2) and PEG, and that H2O2 mediated PEG-induced expression of ZmMKK3. Constitutive expression of ZmMKK3 in Nicotiana tabacum reduced H2O2 accumulation under osmotic stress by affecting antioxidant defense systems and alleviated reactive oxygen species-mediated injury under oxidative stress. Transgenic tobacco exhibited attenuated ABA sensitivity by means of an increased germination rate and main root growth. Taken together, these results indicate that ZmMKK3 is a positive regulator of osmotic tolerance and ABA signaling in plants. (C) 2012 Elsevier GmbH. All rights reserved.

引用

页码：1501 / 1510

页数：10

共 40 条

[1] Reactive oxygen species: Metabolism, oxidative stress, and signal transduction [J].