A NAC transcription factor and SNI1 cooperatively suppress basal pathogen resistance in Arabidopsis thaliana

被引：53

作者：

Kim, Ho Soo ^{[1
,2
]}

Park, Hyeong Cheol ^{[3
]}

Kim, Kyung Eun ^{[1
,3
]}

Jung, Mi Soon ^{[3
]}

Han, Hay Ju ^{[1
]}

Kim, Sun Ho ^{[3
]}

Kwon, Young Sang ^{[1
]}

Bahk, Sunghwa ^{[1
]}

An, Jonguk ^{[1
]}

Bae, Dong Won ^{[4
]}

Yun, Dae-Jin ^{[1
,3
]}

Kwak, Sang-Soo ^{[2
]}

Chung, Woo Sik ^{[1
,3
]}

机构：

[1] Gyeongsang Natl Univ, Div Appl Life Sci, Program BK21, Jinju 660701, South Korea

[2] Korea Res Inst Biosci & Biotechnol, Environm Biotechnol Res Ctr, Taejon 305806, South Korea

[3] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Jinju 660701, South Korea

[4] Gyeongsang Natl Univ, Cent Instrument Facil, Jinju 660701, South Korea

来源：

NUCLEIC ACIDS RESEARCH | 2012年 / 40卷 / 18期

基金：

新加坡国家研究基金会;

关键词：

SYSTEMIC ACQUIRED-RESISTANCE; PLANT DEFENSE RESPONSES; SALICYLIC-ACID; GENE-EXPRESSION; PR-1; GENE; SIGNAL-TRANSDUCTION; DISEASE RESISTANCE; DNA-BINDING; TGA FACTORS; CELL-DEATH;

D O I：

10.1093/nar/gks683

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcriptional repression of pathogen defense-related genes is essential for plant growth and development. Several proteins are known to be involved in the transcriptional regulation of plant defense responses. However, mechanisms by which expression of defense-related genes are regulated by repressor proteins are poorly characterized. Here, we describe the in planta function of CBNAC, a calmodulin-regulated NAC transcriptional repressor in Arabidopsis. A T-DNA insertional mutant (cbnac1) displayed enhanced resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae DC3000 (PstDC3000), whereas resistance was reduced in transgenic CBNAC overexpression lines. The observed changes in disease resistance were correlated with alterations in pathogenesis-related protein 1 (PR1) gene expression. CBNAC bound directly to the PR1 promoter. SNI1 (suppressor of nonexpressor of PR genes1, inducible 1) was identified as a CBNAC-binding protein. Basal resistance to PstDC3000 and derepression of PR1 expression was greater in the cbnac1 sni1 double mutant than in either cbnac1 or sni1 mutants. SNI1 enhanced binding of CBNAC to its cognate PR1 promoter element. CBNAC and SNI1 are hypothesized to work as repressor proteins in the cooperative suppression of plant basal defense.

引用

页码：9182 / 9192

页数：11

共 42 条

[1] Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].

Alonso, JM ;

Stepanova, AN ;

Leisse, TJ ;

Kim, CJ ;

Chen, HM ;

Shinn, P ;

Stevenson, DK ;

Zimmerman, J ;

Barajas, P ;

Cheuk, R ;

Gadrinab, C ;

Heller, C ;

Jeske, A ;

Koesema, E ;

Meyers, CC ;

Parker, H ;

Prednis, L ;

Ansari, Y ;

Choy, N ;

Deen, H ;

Geralt, M ;

Hazari, N ;

Hom, E ;

Karnes, M ;

Mulholland, C ;

Ndubaku, R ;

Schmidt, I ;

Guzman, P ;

Aguilar-Henonin, L ;

Schmid, M ;

Weigel, D ;

Carter, DE ;

Marchand, T ;

Risseeuw, E ;

Brogden, D ;

Zeko, A ;

Crosby, WL ;

Berry, CC ;

Ecker, JR .

SCIENCE, 2003, 301 (5633) :653-657

[2] The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance [J].

Bowling, SA ;

Clarke, JD ;

Liu, YD ;

Klessig, DF ;

Dong, XN .

PLANT CELL, 1997, 9 (09) :1573-1584

[3]

CAO H, 1994, PLANT CELL, V6, P1583, DOI 10.1105/tpc.6.11.1583

[4] Firefly luciferase complementation imaging assay for protein-protein interactions in plants [J].

Chen, Huamin ;

Zou, Yan ;

Shang, Yulei ;

Lin, Huiqiong ;

Wang, Yujing ;

Cai, Run ;

Tang, Xiaoyan ;

Zhou, Jian-Min .

PLANT PHYSIOLOGY, 2008, 146 (02) :368-376

[5] Constitutive disease resistance requires EDS1 in the Arabidopsis mutants cpr1 and cpr6 and is partially EDS1-dependent in cpr5 [J].

Clarke, JD ;

Aarts, N ;

Feys, BJ ;

Dong, XN ;

Parker, JE .

PLANT JOURNAL, 2001, 26 (04) :409-420

[6] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[7] Plant pathogens and integrated defence responses to infection [J].

Dangl, JL ;

Jones, JDG .

NATURE, 2001, 411 (6839) :826-833

[8] ARABIDOPSIS SIGNAL-TRANSDUCTION MUTANT DEFECTIVE IN CHEMICALLY AND BIOLOGICALLY INDUCED DISEASE RESISTANCE [J].

DELANEY, TP ;

FRIEDRICH, L ;

RYALS, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) :6602-6606

[9] The arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors [J].

Després, C ;

DeLong, C ;

Glaze, S ;

Liu, E ;

Fobert, PR .

PLANT CELL, 2000, 12 (02) :279-290

[10] Ca2+/calmodulin regulates salicylic-acid-mediated plant immunity [J].

Du, Liqun ;

Ali, Gul S. ;

Simons, Kayla A. ;

Hou, Jingguo ;

Yang, Tianbao ;

Reddy, A. S. N. ;

Poovaiah, B. W. .

NATURE, 2009, 457 (7233) :1154-U116

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