Demineralized bone matrix gelatin as scaffold for osteochondral tissue engineering

被引：88
作者：
Li, XD
Jin, L
Balian, G
Laurencin, CT
Anderson, DG
机构：
[1] Univ Virginia, Sch Med, Dept Orthopaed Surg, Charlottesville, VA 22908 USA
[2] Univ Virginia, Sch Med, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA
[3] Thomas Jefferson Univ, Dept Orthopaed Surg, Rothman Inst, Philadelphia, PA 19107 USA
来源：
BIOMATERIALS | 2006年 / 27卷 / 11期
关键词：
cartilage tissue engineering; chondrocyte; bone graft; cell culture; collagen;
D O I：
10.1016/j.biomaterials.2005.11.040
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
To develop a single-unit osteochondral tissue with demineralized bone matrix gelatin (BMG), rabbit chondrocytes were cultured on demineralized bone matrix gelatin for 6 weeks. The engineered osteochondral tissue was characterized with histology, immunolocalization, TEM, SEM, biochemical assay, and gene expression analysis. About 1.3 mm viable neo-cartilage was produced on demineralized BMG. RT-PCR, immunohistochemistry, TEM, biochemical assay, and histology revealed hyaline-like cartilage with zonal layers, intense type II collagen expression, and abundant proteoglycan content formed upon BMG compared with normal cartilage. But hydroxyproline content and type I collagen gene and protein expressions were significantly lower. We consider engineering cartilage tissue with chondrocytes cultured on allogenic demineralized BMG is a good approach for osteochondral tissue engineering. (c) 2005 Elsevier Ltd. All rights reserved.
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收藏
页码：2426 / 2433
页数：8
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