Co-production of ice nuclei and xanthan gum by transformed Xanthomonas campestris grown in sugar beet molasses

被引：6

作者：

Afendra, AS ^{[1
]}

Yiannaki, EE

Palaiomylitou, MA

Kyriakidis, DA

Drainas, C

机构：

[1] Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110 Ioannina, Greece

[2] Aristotelian Univ Thessaloniki, Dept Chem, Biochem Lab, GR-54006 Thessaloniki, Greece

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 07期

关键词：

fermentation; ice nucleation gene; molasses; xanthan gum; Xanthomonas campestris;

D O I：

10.1023/A:1014872811167

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two recombinant plasmids, expressing ice nucleation activity, were constructed and named pCPP30inaZ and pCPP38inaZ. They were transferred to the ice-negative, xanthan-producing Xanthomonas campestris pv. campestris by electroporation. The transformants were used for co-production of xanthan gum and ice nuclei from sugar beet molasses. The highest values obtained were 20 g l(-1) and 10(18) ice nuclei ml(-1), respectively. The above values fulfil the criteria for industrial manipulation. This is the first report on co-formation of two products by a transformed X. campestris strain.

引用

页码：579 / 583

页数：5

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