BmNHR96 participate BV entry of BmN-SWU1 cells via affecting the cellular cholesterol level

被引：16

作者：

Dong, Xiao-long ^{[1
,3
]}

Liu, Tai-hang ^{[1
]}

Wang, Wei ^{[1
]}

Pan, Cai-xia ^{[1
]}

Du, Guo-yu ^{[1
]}

Wu, Yun-fei ^{[1
]}

Pan, Min-hui ^{[1
,2
]}

Lu, Cheng ^{[1
,2
]}

机构：

[1] Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400716, Peoples R China

[2] Southwest Univ, Minist Agr, Key Lab Sericulture Funct Genom & Biotechnol, Chongqing, Peoples R China

[3] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 482卷 / 04期

基金：

中国国家自然科学基金;

关键词：

BmNHR96; BV infection; Cholesterol; Bombyx mori; NUCLEOPOLYHEDROVIRUS GP64; MEMBRANE-FUSION; RECEPTOR; BACULOVIRUS; PROTEIN;

D O I：

10.1016/j.bbrc.2016.12.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

B.mori nucleopolyhedrovirus (BmNPV), which produces BV and ODV two virion phenotypes in its life cycle, caused the amount of economic loss in sericulture. But the mechanism of its infection was still unclear. In this study we characterized B.mori nuclear hormone receptor 96 (BmNHR96) as a NHR96 family member, which was localized in the nucleus. We also found BmNHR96 over-expression could enhance the entry of BV as well as cellular cholesterol level. Furthermore, we validated that BmNHR96 increased membrane fusion mediated by GP64, which could probably promote BV-infection. In summary, our study suggested that BmNHR96 plays an important role in BV infection and this function probably actualized by affecting cellular cholesterol level, and our results provided insights to the mechanisms of By-infection of B.mori. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：1484 / 1490

页数：7

共 21 条

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