Occurrence of Natural Vertical Transmission of Dengue-2 and Dengue-3 Viruses in Aedes aegypti and Aedes albopictus in Fortaleza, Ceara, Brazil

被引：53
作者：
Pessoa Martins, Victor Emanuel [1 ]
Alencar, Carlos Henrique [2 ]
Kamimura, Michel Tott [3 ]
de Carvalho Araujo, Fernanda Montenegro [4 ]
De Simone, Salvatore Giovanni [5 ]
Dutra, Rosa Fireman [6 ]
Florindo Guedes, Maria Izabel [1 ]
机构：
[1] Univ Estadual Ceara, Human Biochem Lab, Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, Dept Community Hlth, Fortaleza, Ceara, Brazil
[3] Univ Estadual Ceara, Ctr Genom & Bioinformat, Fortaleza, Ceara, Brazil
[4] Ceara State Hlth Secretariat, Cent Lab Publ Hlth, Fortaleza, Ceara, Brazil
[5] Inst Oswaldo Cruz FIOCRUZ, Peptides & Prot Lab, Rio De Janeiro, Brazil
[6] Univ Fed Pernambuco, Biomed Engn Lab, Recife, PE, Brazil
来源：
PLOS ONE | 2012年 / 7卷 / 07期
关键词：
TRANS-OVARIAL TRANSMISSION; POLYMERASE-CHAIN-REACTION; TRANSOVARIAL TRANSMISSION; STEGOMYIA ALBOPICTUS; STRAINS; DIPTERA; STATE; CULICIDAE; SUSCEPTIBILITY; SURVEILLANCE;
D O I：
10.1371/journal.pone.0041386
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Background: Aedes aegypti and Aedes albopictus perform an important role in the transmission of the dengue virus to human populations, particularly in the tropical and subtropical regions of the world. Despite a lack of understanding in relation to the maintenance of the dengue virus in nature during interepidemic periods, the vertical transmission of the dengue virus in populations of A. aegypti and A. albopictus appears to be of significance in relation to the urban scenario of Fortaleza. Methods: From March 2007 to July 2009 collections of larvae and pupae of Aedes spp were carried out in 40 neighborhoods of Fortaleza. The collections yielded 3,417 (91%) A. aegypti mosquitoes and 336 (9%) A. albopictus mosquitoes. Only pools containing females, randomly chosen, were submitted to the following tests indirect immunofluorescence (virus isolation), RT-PCR/nested-PCR and nucleotide sequencing at the C-prM junction of the dengue virus genome. Results: The tests on pool 34 (35 A. albopictus mosquitoes) revealed with presence of DENV-3, pool 35 (50 A. aegypti mosquitoes) was found to be infected with DENV-2, while pool 49 (41 A. albopictus mosquitoes) revealed the simultaneous presence of DENV-2 and DENV-3. Based on the results obtained, there was a minimum infection rate of 0.5 for A. aegypti and 9.4 for A. albopictus. The fragments of 192 bp and 152 bp related to DENV-3, obtained from pools 34 and 49, was registered in GenBank with the access codes HM130699 and JF261696, respectively. Conclusions: This study recorded the first natural evidence of the vertical transmission of the dengue virus in populations of A. aegypti and A. albopictus collected in Fortaleza, Ceara State, Brazil, opening a discuss on the epidemiological significance of this mechanism of viral transmission in the local scenario, particularly with respect to the maintenance of these viruses in nature during interepidemic periods.
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相关论文
共 63 条
[31]   Global Spread and Persistence of Dengue [J].
Kyle, Jennifer L. ;
Harris, Eva .
ANNUAL REVIEW OF MICROBIOLOGY, 2008, 62 :71-92
[32]   Consequences of the Expanding Global Distribution of Aedes albopictus for Dengue Virus Transmission [J].
Lambrechts, Louis ;
Scott, Thomas W. ;
Gubler, Duane J. .
PLOS NEGLECTED TROPICAL DISEASES, 2010, 4 (05)
[33]   RAPID DETECTION AND TYPING OF DENGUE VIRUSES FROM CLINICAL-SAMPLES BY USING REVERSE TRANSCRIPTASE-POLYMERASE CHAIN-REACTION [J].
LANCIOTTI, RS ;
CALISHER, CH ;
GUBLER, DJ ;
CHANG, GJ ;
VORNDAM, AV .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (03) :545-551
[34]  
Lee H. L., 2005, Dengue Bulletin, V29, P106
[35]   Aedes aegypti in Brazil:: genetically differentiated populations with high susceptibility to dengue and yellow fever viruses [J].
Lourenço-De-Oliveira, R ;
Vazeille, M ;
de Filippis, AMB ;
Failloux, AB .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2004, 98 (01) :43-54
[36]   Dengue virus type 3 isolation from Aedes aegypti in the municipality of Nova Iguacu, State of Rio de Janeiro [J].
Lourenço-de-Oliveira, R ;
Honório, NA ;
Castro, MG ;
Schatzmayr, HG ;
Miagostovich, MP ;
Alves, JCR ;
Silva, WC ;
Leite, PJ ;
Nogueira, RMR .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 (06) :799-800
[37]  
Mardihusodo SJ, 2008, EPIDEMIOL MOL EVOL, P297
[38]   Diagnosis of dengue by using reverse transcriptase-polymerase chain reaction [J].
Miagostovich, MP ;
dosSantos, FB ;
deAraujo, ESM ;
Dias, J ;
Schatzmayr, HG ;
Nogueira, RMR .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1997, 92 (05) :595-599
[39]  
MITCHELL CJ, 1990, J AM MOSQUITO CONTR, V6, P251
[40]   Aedes albopictus in the United States: Ten-year presence and public health implications [J].
Moore, CG ;
Mitchell, CJ .
EMERGING INFECTIOUS DISEASES, 1997, 3 (03) :329-334
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