Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae

被引：49
作者：
Han, Hui [1 ]
Zhou, Haijian [2 ,3 ]
Li, Haishan [1 ]
Gao, Yuan [2 ,3 ]
Lu, Zhi [4 ]
Hu, Kongxin [1 ]
Xu, Baoliang [1 ]
机构：
[1] Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China
[2] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China
[3] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China
[4] Gaoling Ctr Dis Control & Prevent, Xian 710200, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH | 2013年 / 10卷 / 07期
关键词：
Klebsiella pneumoniae; pulsed-field gel electrophoresis; molecular subtyping; RESISTANT STAPHYLOCOCCUS-AUREUS; LIVER-ABSCESS; ESCHERICHIA-COLI; TYPING SYSTEMS; PROTOCOL; SPREAD; ENTEROBACTERIACEAE; SURVEILLANCE; VALIDATION; STRAINS;
D O I：
10.3390/ijerph10072720
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A total of 110 strains of Klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal EP for XbaI PFGE of K. pneumoniae. By software analysis and pilot study, AvrII was chosen as another PFGE enzyme. Both XbaI-and AvrII-PFGE gave D-values higher than 0.99 for 69 K. pneumoniae isolated from different sources. Our results also showed good typeability, reproducibility of both XbaI-and AvrII-PFGE for K. pneumoniae subtyping. Furthermore, the established PFGE method also had good discriminatory power to distinguish outbreak K. pneumoniae strains and a high degree of consistency with multilocus sequence typing method. A rapid PFGE protocol was established here, which could be used for genotyping and other researches of K. pneumoniae.
引用
收藏
页码：2720 / 2731
页数：12
相关论文
共 25 条
  • [1] Rapid spread of carbapenem-resistant Klebsiella pneumoniae in New York City -: A new threat to our antibiotic armamentarium
    Bratu, S
    Landman, D
    Haag, R
    Recco, R
    Eramo, A
    Alam, M
    Quale, J
    [J]. ARCHIVES OF INTERNAL MEDICINE, 2005, 165 (12) : 1430 - 1435
  • [2] Development and validation of a PulseNet standardized pulsed-field gel electrophoresis protocol for subtyping of Vibrio cholerae
    Cooper, K. L. F.
    Luey, C. K. Y.
    Bird, M.
    Terajima, J.
    Nair, G. B.
    Kam, K. M.
    Arakawa, E.
    Safa, A.
    Cheung, D. T.
    Law, C. P.
    Watanabe, H.
    Kubota, K.
    Swaminathan, B.
    Ribot, E. M.
    [J]. FOODBORNE PATHOGENS AND DISEASE, 2006, 3 (01) : 51 - 58
  • [3] Worldwide Diversity of Klebsiella pneumoniae That Produces β-Lactamase blaKPC-2 Gene
    Cuzon, Gaelle
    Naas, Thierry
    Truong, HaVy
    Villegas, Maria-Virginia
    Wisell, Karin T.
    Carmeli, Yehuda
    Gales, Ana C.
    Navon-Venezia, Shiri
    Quinn, John P.
    Nordmann, Patrice
    [J]. EMERGING INFECTIOUS DISEASES, 2010, 16 (09) : 1349 - 1356
  • [4] Occurrence and characterization of carbapenemase-producing Enterobacteriaceae: Report from the SENTRY Antimicrobial Surveillance Program (2000-2004)
    Deshpande, Lalitagauri M.
    Jones, Ronald N.
    Fritsche, Thomas R.
    Sader, Helio S.
    [J]. MICROBIAL DRUG RESISTANCE, 2006, 12 (04) : 223 - 230
  • [5] Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates
    Diancourt, L
    Passet, V
    Verhoef, J
    Grimont, PAD
    Brisse, S
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) : 4178 - 4182
  • [6] A global emerging disease of Klebsiella pneumoniae liver abscess:: is serotype K1 an important factor for complicated endophthalmitis?
    Fung, CP
    Chang, FY
    Lee, SC
    Hu, BS
    Kuo, BIT
    Liu, CY
    Ho, M
    Siu, LK
    [J]. GUT, 2002, 50 (03) : 420 - 424
  • [7] NUMERICAL INDEX OF THE DISCRIMINATORY ABILITY OF TYPING SYSTEMS - AN APPLICATION OF SIMPSONS INDEX OF DIVERSITY
    HUNTER, PR
    GASTON, MA
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (11) : 2465 - 2466
  • [8] Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: Converting the national databases to the new size standard
    Hunter, SB
    Vauterin, P
    Lambert-Fair, MA
    Van Duyne, MS
    Kubota, K
    Graves, L
    Wrigley, D
    Barrett, T
    Ribot, E
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (03) : 1045 - 1050
  • [9] Institut Pasteur, KLEBS PNEUM MLST DAT
  • [10] Discrimination of Klebsiella pneumoniae and Klebsiella oxytoca phylogenetic groups and other Klebsiella species by use of amplified fragment length polymorphism
    Jonas, D
    Spitzmüller, B
    Daschner, FD
    Verhoef, J
    Brisse, S
    [J]. RESEARCH IN MICROBIOLOGY, 2004, 155 (01) : 17 - 23
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