Digital PCR strategies in the development and analysis of molecular biomarkers for personalized medicine

被引：143
作者：
Day, Elizabeth [1 ]
Dear, Paul H. [1 ]
McCaughan, Frank [1 ,2 ,3 ]
机构：
[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[3] Kings Coll London, Dept Resp Med & Allergy, London WC2R 2LS, England
来源：
METHODS | 2013年 / 59卷 / 01期
基金：
英国医学研究理事会;
关键词：
Digital PCR; Single molecule; Biomarkers; POLYMERASE-CHAIN-REACTION; COPY NUMBER VARIATION; ABL TYROSINE KINASE; GENOME AMPLIFICATION; DNA METHYLATION; GENE-EXPRESSION; T790M MUTATION; CANCER; QUANTIFICATION; QUANTITATION;
D O I：
10.1016/j.ymeth.2012.08.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The efficient delivery of personalized medicine is a key goal of healthcare over the next decade. It is likely that PCR strategies will play an important role in the delivery of this goal. Digital PCR has certain advantages over more traditional PCR protocols. In this article we will discuss the current status of digital PCR, highlighting its advantages and focusing on how it can be utilized in biomarker development and analysis, including the use of individualized biomarkers. We will explore recent developments in this field including examples of how digital PCR may integrate with next generation sequencing to deliver truly personalized medicine. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：101 / 107
页数：7
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