Detection of the Wolbachia-encoded DNA binding protein, HU beta, in mosquito gonads

被引：17

作者：

Beckmann, John F. ^{[1
]}

Markowski, Todd W. ^{[2
]}

Witthuhn, Bruce A. ^{[2
]}

Fallon, Ann M. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA

[2] Univ Minnesota, Ctr Mass Spectrometry & Prote, St Paul, MN 55108 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2013年 / 43卷 / 03期

关键词：

Wolbachia; Cytoplasmic incompatibility; HU; Culex pipiens; Mosquito; SDS PAGE; CYTOPLASMIC INCOMPATIBILITY; STATISTICAL-MODEL; CHROMOSOME; PIPIENTIS; IHF; SPERMATOGENESIS; CHROMATIN; ACCURACY; SEQUENCE; INSECT;

D O I：

10.1016/j.ibmb.2012.12.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wolbachia are obligate intracellular bacteria that cause cytoplasmic incompatibility in mosquitoes. In an incompatible cross, eggs of uninfected females fail to hatch when fertilized by sperm from infected males. We used polyacrylamide gel electrophoresis and tandem mass spectrometry to identify Wolbachia proteins in infected mosquito gonads. These included surface proteins with masses of 25 and 18 kDa and the DNA binding protein, HU beta. Using reverse transcriptase polymerase chain reaction, we showed that the HU gene is transcribed in Wolbachia-infected Culex pipiens and Aedes albopictus mosquitoes. We sequenced HU genes from four Wolbachia strains and compared deduced protein sequences with additional homologs from the databases. Among the Rickettsiales, Wolbachia HU has distinct N- and C-terminal basic/acidic amino acid motifs as well as a pair of conserved, cysteine residues. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：272 / 279

页数：8

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