Interaction of Na2B12H11SH with dimyristoyl phosphatidylcholine liposomes

被引：27

作者：

Awad, Doaa ^{[1
,2
]}

Damian, Luminita ^{[3
]}

Winterhalter, Mathias ^{[3
]}

Karlsson, Goeran ^{[4
]}

Edwards, Katarina ^{[4
]}

Gabel, Detlef ^{[1
]}

机构：

[1] Univ Bremen, Dept Chem, D-28334 Bremen, Germany

[2] Univ Alexandria, Fac Sci, Dept Biochem, Alexandria 21511, Egypt

[3] Jacobs Univ Bremen, Sch Sci & Engn, D-28759 Bremen, Germany

[4] Uppsala Univ, Dept Phys & Analyt Chem, S-75123 Uppsala, Sweden

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2009年 / 157卷 / 02期

基金：

瑞典研究理事会;

关键词：

Boranes; Electron microscopy; Calorimetry; SOLUBILIZATION; CALORIMETRY; TRANSITION; SURFACTANT; VESICLES;

D O I：

10.1016/j.chemphyslip.2008.11.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous investigations have revealed that the boron cluster Compound Na2B12H11SH (BSH) is very potent in causing major structural rearrangements of and leakage from phosphatidylcholine liposomes. This somewhat unexpected finding is interesting from a fundamental point of view and may also constitute the basis of future important pharmaceutical/medical applications of BSH. In order to further explore the BSH-lipid interaction, we have Studied the effects Caused by BSH on dimyristoyl phosphatidylcholine (DMPC) liposomes. Cryo-transmission electron microscopy showed that BSH induces aggregation, membrane rupture and increasing wall thickness of the liposomes. Differential scanning calorimetry revealed a BSH dependent shift of the gel to liquid crystalline phase transition temperature of DMPC. The zeta potential of the liposomes decreases with increasing BSH concentrations, and an apparent dissociation constant of 0.23 mM Was found. BSH Caused leakage of liposome-encapsulated carboxyfluorescein; leakage was higher at 23 C (near the phase transition temperature) than at 15 C and 37 C. It induced lipid mixing only at very high concentrations. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：78 / 85

页数：8

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