A new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules

被引：101

作者：

Gu, Xinggui ^{[1
]}

Zhang, Guanxin ^{[1
]}

Wang, Zhuo ^{[2
]}

Liu, Wenwen ^{[2
]}

Xiao, Le ^{[2
]}

Zhang, Deqing ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Chem, Organ Solids Lab, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Natl Ctr Nanosci & Technol, Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100080, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 08期

关键词：

INDUCED EMISSION CHARACTERISTICS; FLUORESCENCE DETECTION; ACETYLCHOLINESTERASE ACTIVITY; PROBE; SILOLE; SERUM; ENHANCEMENT; CONVENIENT; SUBSTRATE; HEPARIN;

D O I：

10.1039/c3an36784c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on the consideration that compound 1 with a -PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL(-1) can be assayed with compound 1. Moreover, compound 1 has been successfully applied for ALP assay in living cells. Also, compound 1 is useful for screening inhibitors of ALP.

引用

页码：2427 / 2431

页数：5

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