Amino acid sequence identification and anti-inflammatory potency evaluation of dandelion oligopeptides in LPS-induced RAW264.7 macrophage cells

被引：4
作者：
Lai, Jicai [1 ]
Peng, Lizhi [1 ]
Chen, Hongda [2 ]
Li, Ruiming [1 ]
Liu, Liuyi [1 ]
Liu, Jie [3 ]
Shi, Xianfang [1 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 7, Shenzhen 518107, Peoples R China
[2] Guangzhou Univ Chinese Med, Guangzhou 510006, Peoples R China
[3] Shenzhen Univ, Affiliated Hosp 3, Shenzhen 518020, Peoples R China
来源：
JOURNAL OF MOLECULAR STRUCTURE | 2020年 / 1216卷
关键词：
Dandelion; Inflammation; MAPK; PI3K; Macrophage cells; ACTIVATION; PATHWAYS; KINASES;
D O I：
10.1016/j.molstruc.2020.128280
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The dandelion is a famous traditional Chinese medicine. However, little research was performed on the anti-in fl ammatory potency evaluation of dandelion oligopeptides (DOPs). In current study, we isolated the DOPs and explored the anti-in fl ammatory potency of them on LPS-induced macrophages. The results indicated that DOP-1 effectively inhibited LPS-induced in fl ammatory cytokines over-expression. Signal transduction studies showed that DOP-1 may repress the phosphorylation of p38, ERK1/2, JNK, phosphatidylinositol 3-kinase (PI3K) and Akt, and inhibits the activation of nuclear factor-kappa B (NF kappa B). Of note, our investigation suggests DOPs are potential anti-inflammatory bioactive oligopeptides and further researches were on the way. (c) 2020 Elsevier B.V. All rights reserved.
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页数：8
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