Fluorescence correlation spectroscopy in membrane structure elucidation

被引：115
作者：
Chiantia, Salvatore [1 ]
Ries, Jonas [1 ]
Schwille, Petra [1 ]
机构：
[1] Tech Univ Dresden, Biotechnol Zentrum BIOTEC, Dresden, Germany
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2009年 / 1788卷 / 01期
关键词：
Membrane dynamics; FCS; Anomalous diffusion; Raft; Picket model; Fence model; LIQUID-ORDERED DOMAINS; SINGLE-MOLECULE FLUORESCENCE; ATOMIC-FORCE MICROSCOPY; UNDERGO HOP DIFFUSION; LIPID RAFTS; PLASMA-MEMBRANE; LATERAL DIFFUSION; MODEL MEMBRANES; COMBINED AFM; CONFOCAL FLUORESCENCE;
D O I：
10.1016/j.bbamem.2008.08.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This review describes the application of fluorescence correlation spectroscopy (FCS) for the study of biological membranes. Monitoring the fluorescence signal fluctuations, it is possible to obtain diffusion constants and concentrations for several membrane components. Focusing the attention on lipid bilayers, we explain the technical difficulties and the new FCS-based methodologies introduced to overcome them. Finally, we report several examples of studies which apply FCS on both model and biological membranes to obtain interesting insight in the topic of lateral membrane organization. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：225 / 233
页数：9
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