Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy

被引：5

作者：

Wang, Li ^{[1
]}

Li, Jintao ^{[2
]}

Zhang, Na ^{[2
]}

Zhang, Xiaofei ^{[1
]}

Xia, Yang ^{[2
]}

Chai, Binbin ^{[2
]}

Gao, Chunlang ^{[1
]}

Mao, Shengcheng ^{[1
]}

Ji, Yuan ^{[1
]}

Sheng, Wang ^{[2
]}

Han, Xiaodong ^{[1
]}

机构：

[1] Beijing Univ Technol, Inst Microstruct & Property Adv Mat, Beijing Key Lab Microstruct & Properties Solids, Beijing 100124, Peoples R China

[2] Beijing Univ Technol, Cooperat Base Antivirus Drug, Beijing Key Lab Environm & Viral Oncol, Coll Life Sci & Bioengn,Beijing Int Sci & Technol, Beijing 100124, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 532卷 / 02期

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

EGFR; Configuration; Oligomerization; TEM; SEM; Structural model; EPIDERMAL-GROWTH-FACTOR; FACTOR RECEPTOR EGFR; CRYSTAL-STRUCTURE; ACTIVATION; EXPRESSION; AFFINITY; LIMIT;

D O I：

10.1016/j.bbrc.2020.07.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epidermal growth factor receptor (EGFR) is an important target for tumor therapy in various tumors. The current understanding of EGFR conformations on the cell surface is based on X-ray structural data, molecular dynamic simulations, and fluorescence-localization imaging. Using scanning electron microscope (SEM) and transmission electron microscope (TEM) with the resolution at sub-nanometers, we successfully recognized individual molecules of EGFRs and their assembly details on the surface of triple-negative breast cancer (TNBC) upon one-to-one labeling by Au nanoparticles. Based on our results, we have proposed the possible configurations, structural models, and conformational transitions of EGFR oligomers. Our study shows that the high-resolution electron imaging is an invaluable tool to provide direct evidence of EGFR configuration on tumor cell surfaces, and may play a pivotal role in further understanding of EGFR-associated signaling and tumor therapy. (C) 2020 Elsevier Inc. All rights reserved.

引用

页码：179 / 184

页数：6

共 31 条

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