Two new chiral EDTA-based metal chelates for weak alignment of proteins in solution

被引：41

作者：

Haberz, P ^{[1
]}

Rodriguez-Castañeda, F ^{[1
]}

Junker, J ^{[1
]}

Becker, S ^{[1
]}

Leonov, A ^{[1
]}

Griesinger, C ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany

来源：

ORGANIC LETTERS | 2006年 / 8卷 / 07期

关键词：

D O I：

10.1021/ol053049o

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue of a protein via a disuffide bond is described. The complexes were used after coordination of lanthanides to align trigger factor and apo-calmodulin in solution to yield residual dipolar couplings and pseudocontact shifts. Alignment tensors for the new tags are linearly independent compared to those of previously published tags.

引用

页码：1275 / 1278

页数：4

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