Improved methods for encoding and decoding dialkylamine-encoded combinatorial libraries

被引：32
作者：
Fitch, WL
Baer, TA
Chen, WW
Holden, F
Holmes, CP
Maclean, D
Shah, NH
Sullivan, E
Tang, M
Waybourn, P
Fischer, SM
Miller, CA
Synder, LR
机构：
[1] Affymax Res Inst, Santa Clara, CA 95051 USA
[2] Hewlett Packard Co, Palo Alto, CA 94304 USA
[3] LC Resources Inc, Orinda, CA 94563 USA
来源：
JOURNAL OF COMBINATORIAL CHEMISTRY | 1999年 / 1卷 / 03期
关键词：
D O I：
10.1021/cc990001r
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Optimized methods for utilizing and analyzing dialkylamine tags in encoded combinatorial chemistry are reported. A previously described LC/fluorescence method has been shortened from over 1 h to 6 min. A novel ion exchange LC/MS method for decoding has been developed which is both fast and rugged. The use of quantitative encoding schemes is described. These new techniques have allowed a simplified, automated sample preparation scheme, and over 300 encoded bead samples can be read in a day.
引用
收藏
页码：188 / 194
页数：7
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