Qualitative and Quantitative Proteomic Profiling of Cripto-/- Embryonic Stem Cells by Means of Accurate Mass LC-MS Analysis

被引：16
作者：
Chambery, Angela [1 ,2 ]
Vissers, Johannes P. C.
Langridge, James I.
Lonardo, Enza [3 ]
Minchiotti, Gabriella [3 ]
Ruvo, Menotti [4 ]
Parente, Augusto [1 ,2 ]
机构：
[1] Univ Naples 2, Dipartimento Sci Vita, I-81100 Caserta, Italy
[2] Waters Corp, MS Technol Ctr, Manchester M22 5PP, Lancs, England
[3] CNR, Ist Genet & Biofis A Buzzati Traverso, I-80131 Naples, Italy
[4] CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy
来源：
JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 02期
关键词：
Cripto; LC-MS; proteomic profiling; mass spectrometry; data independent scanning; Hsp25; HEAT-SHOCK-PROTEIN; IDENTIFICATION TECHNOLOGY; NEURONAL DIFFERENTIATION; MOLECULAR CHAPERONES; HISTONE ACETYLATION; GENE-EXPRESSION; INTACT PROTEINS; MOUSE EMBRYO; GROWTH; HSP25;
D O I：
10.1021/pr800485c
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto I murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were used to qualitatively and quantitatively profile the cripto(-/-) ESC proteome, providing an integral view of the alterations induced in stem cell functions by deleting the cripto gene.
引用
收藏
页码：1047 / 1058
页数：12
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