Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase (blaKPC) and New Delhi Metallo-β-Lactamase (blaNDM) in Gram-Negative Bacilli

被引：24

作者：

Cunningham, Scott A. ^{[1
]}

Noorie, Tabassum ^{[2
]}

Meunier, Daniele ^{[2
]}

Woodford, Neil ^{[2
]}

Patel, Robin ^{[1
,3
]}

机构：

[1] Mayo Clin, Dept Lab Med & Pathol, Div Clin Microbiol, Rochester, MN 55905 USA

[2] Hlth Protect Agcy HPA, Antimicrobial Resistance & Healthcare Associated, London, England

[3] Mayo Clin, Div Infect Dis, Dept Internal Med, Rochester, MN USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 04期

关键词：

REAL-TIME PCR; CONVENIENT TEST; RESISTANCE; ENTEROBACTERIACEAE; MICROARRAY; BACTERIA; KPC;

D O I：

10.1128/JCM.03062-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (bla(KPC)) and New Delhi metallo-beta-lactamase (bla(NDM)) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

引用

页码：1269 / 1271

页数：3

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