Expression and role of miR-34a in bladder cancer

被引：0

作者：

Wang, Wei ^{[1
]}

Li, Tan ^{[2
]}

Han, Guang ^{[1
]}

Li, Ying ^{[1
]}

Shi, Li-hua ^{[1
]}

Li, Hui ^{[1
]}

机构：

[1] Logist Univ Chinese Peoples Armed Police Force, Affiliated Hosp, Dept Urol, Tianjin 300162, Peoples R China

[2] Logist Univ Chinese Peoples Armed Police Force, Dept Pathogen Biol & Immunol, Tianjin 300162, Peoples R China

来源：

INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | 2013年 / 50卷 / 02期

关键词：

microRNA-34a; Bladder cancer; Pathological variables; Proliferation; Apoptosis; MICRORNA; PROLIFERATION;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the biological function of microRNA-34a (miR-34a) in bladder cancer, the expression of miR-34a was determined using quantitative real-time polymerase chain (qRT-PCR) reaction in 42 cases of bladder cancer. The relationship between the expression of miR-34a and development of bladder cancer was also studied. The mature mimics of miR-34a were chemically synthesized and transiently transfected into human bladder cancer T24 cells. The effects of miR-34a on apoptosis, cell cycle and proliferation in T24 cells were evaluated by flow cytometry and MTT, respectively. The results showed that the low expression rate of miR-34a was correlated with the malignancy and tumor size of bladder cancer. The up-regulation of miR-34a in T24 cells contributed to cell growth and cell cycle arrest, but not caspase-3 pathway. These findings suggest that the relative low expression of miRNA-34a might be involved in the tumorigenesis of bladder cancer.

引用

页码：87 / 92

页数：6

共 21 条

[1] The Tumor Suppressors p53, p63, and p73 Are Regulators of MicroRNA Processing Complex [J].