Integrated process of extraction and purification of betanin from Opuntia ficus-indica using aqueous two-phase systems based on THF and sodium salts

被引：11

作者：

Santos, Ricardo P. ^{[1
]}

Souza, Luana M. ^{[1
]}

Balieiro, Acenini L. ^{[2
]}

Soares, Cleide M. F. ^{[1
,2
]}

Lima, Alvaro S. ^{[1
,2
]}

Souza, Ranyere L. ^{[1
,2
]}

机构：

[1] Univ Tiradentes, Proc Engn, Aracaju, SE, Brazil

[2] Inst Tecnol & Pesquisa ITP LPA, Aracaju, SE, Brazil

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2018年 / 53卷 / 05期

关键词：

Opuntia ficus-indica; betanin; extraction; purification; aqueous two-phase systems; NATURAL ANTIOXIDANTS; ASSISTED EXTRACTION; PROTEIN SEPARATION; BIPHASIC SYSTEMS; BETALAINS; PLANTS; TETRAHYDROFURAN; OPTIMIZATION; ATTRIBUTES; PARAMETERS;

D O I：

10.1080/01496395.2017.1397022

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This research present an integrated process for the extraction and purification of betanin from fruit of Opuntia ficus-indica. The extraction process was evaluated using different solvents (water, acetonitrile, ethanol and tetrahydrofuran), and then integrated into the purification process using aqueous two-phase systems (ATPS). The optimum conditions for extraction was with THF and the purification conditions determined to be 43 wt% of THF and 6 wt% of sodium citrate buffer (pH 5.5) at 25 degrees C. A purification factor of 13.7 +/- 0.5 fold, with a partition coefficient of betanin for salt-rich phase and the antioxidant capacity was increased approximate to 65%.

引用

页码：734 / 744

页数：11

共 42 条

[21] Isotope ratio mass spectrometrical analysis of betanin and isobetanin isolates for authenticity evaluation of purple pitaya-based products [J].