Determination of telmisartan in human blood plasma Part I:: Immunoassay development

被引：16

作者：

Hempen, C

Gläsle-Schwarz, L

Kunz, U

Karst, U

机构：

[1] Univ Twente, Chem Anal Grp, NL-7500 AE Enschede, Netherlands

[2] Univ Twente, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[3] Boehringer Ingelheim Pharma GmbH & Co KG, Drug Metab & Pharmacokinet, D-88397 Biberach, Germany

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 560卷 / 1-2期

关键词：

telmisartan; ELISA; MNBDH; glucose oxidase; MNBDA;

D O I：

10.1016/j.aca.2005.12.036

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Telmisartan is an angiotensin II receptor antagonist and a known drug against high blood pressure. In this report, the development of anew and rapid analytical technique, an enzyme-linked immunosorbent assay (ELISA) for the determination of telmisartan in human blood plasma is described. The immunoassay is based on a conversion of 4-(N-methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH) to 4-(N-methylamino)-7-nitro2,1,3-benzooxadiazole (MNBDA), which is detected by fluorescence spectroscopy. The limit of detection was 0.1 ng/mL, the limit of quantification was 0.3 ng/mL and the working range extended from 0.3 ng/mL to 300 ng/mL. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：35 / 40

页数：6

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