The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs

被引：57

作者：

Sun, Zhenfei ^{[1
]}

Guo, Tongtong ^{[1
]}

Liu, Yin ^{[1
]}

Liu, Qi ^{[1
]}

Fang, Yuda ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China

来源：

PLOS GENETICS | 2015年 / 11卷 / 10期

基金：

中国国家自然科学基金;

关键词：

NUCLEAR DICING BODIES; BINDING-PROTEIN; FLOWER DEVELOPMENT; DOMAIN PROTEINS; GENE; PLANTS; BIOGENESIS; MATURATION; FAMILY; EXPRESSION;

D O I：

10.1371/journal.pgen.1005598

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The precise regulation of microRNA (miRNA) transcription and processing is important for eukaryotic development. Plant miRNAs are first transcribed as stem-loop primary miRNAs (pri-miRNAs) by RNA polymerase II, then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1). We identified a cycling DOF transcription factor, CDF2, which interacts with DCL1 and regulates the accumulation of a population of miRNAs. CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes. CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. Genetically, CDF2 works in the same pathway as miR156 or miR172 to control flowering. We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.

引用

页数：19

共 53 条

[1] Specialization and evolution of endogenous small RNA pathways [J].