Immobilization of the urease on eggshell membrane and its application in biosensor

被引：47

作者：

D'Souza, S. F. ^{[1
]}

Kumar, Jitendra ^{[1
]}

Jha, Sandeep Kumar ^{[1
]}

Kubal, B. S. ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr Trombay, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2013年 / 33卷 / 02期

关键词：

Polyethyleneimine; Eggshell membrane; Urease; Potentiometric urea biosensor; OPTICAL MICROBIAL BIOSENSOR; ONION BULB SCALE; ENZYME ELECTRODE; GLUCOSE-OXIDASE; INNER EPIDERMIS; COTTON THREADS; CELLS; POLYETHYLENIMINE; PLATFORM; SUCROSE;

D O I：

10.1016/j.msec.2012.11.010

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Eggshell membrane is a natural material, essentially made up of protein fibers having flexibility in the aqueous solution and possessing gas and water permeability. It is used as a biomembrane for immobilization of urease for the development of a potentiometric urea biosensor. Eggshell membrane was treated with polyethyleneimine (PEI) to impart polycation characteristics. Urease was immobilized on the PEI treated eggshell membrane through adsorption. SEM study was carried out to observe the changes in surface morphology after immobilization. FTIR study of membrane was carried out to observe the changes in IR spectra after immobilization of enzyme. Immobilized membrane was associated with ammonium ion selective electrode. Biosensor exhibited sigmoidal responses for the urea concentration range from 0.5 to 10 mM. The response time of the biosensor was 120 s. A single membrane was raised for 270 reactions without loss of activity. The urease-eggshell membranes were stable for 2 months when stored in buffer even at room temperature. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：850 / 854

页数：5

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