1,25-Dihydroxy vitamin D3 treatment attenuates osteopenia, and improves bone muscle quality in Goto-Kakizaki type 2 diabetes model rats

被引：13

作者：

Liang, Yanlong ^{[1
]}

Liu, Yanzhi ^{[1
,2
]}

Lai, Wenxiu ^{[1
]}

Du, Minqun ^{[1
]}

Li, Shuhui ^{[1
]}

Zhou, Limin ^{[1
]}

Mo, Yulin ^{[1
]}

Wang, Pan ^{[1
]}

Min, Yalin ^{[1
]}

Cui, Liao ^{[1
]}

机构：

[1] Guangdong Med Univ, Dept Pharmacol, Guangdong Key Lab Res & Dev Nat Drugs, Zhanjiang 524023, Peoples R China

[2] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Translat Med R&D Ctr, Shenzhen 518055, Peoples R China

来源：

ENDOCRINE | 2019年 / 64卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Type; 2; diabetes; Vitamin D3; Bone; Osteopenia; Calcitriol; GK rat; INSULIN SENSITIVITY; GLUCOSE-METABOLISM; D SUPPLEMENTATION; OXIDATIVE STRESS; CALCIUM; FRACTURES; STRENGTH;

D O I：

10.1007/s12020-019-01857-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Purpose: Osteopenia and skeletal fragility are considered to be the complications associated with type 2 diabetes mellitus (T2DM). The relationship between glucose metabolism, skeletal quality, and vitamin D have not been completely understood. We aimed to demonstrate a comprehensive bone quality profile in a T2DM model subject and to investigate whether 1, 25-dihydroxy vitamin D3 could prevent osteopenia and skeletal fragility in the diabetes model rats. Methods: Daily calcitriol (a 1, 25-dihydroxy vitamin D3 formulation, 0.045g/kg/day) treatment was administered to 21-week-old male Goto-Kakizaki (GK) rats (a genetic non-obese and non-insulin-dependent spontaneous diabetes rat model) for 20 weeks and the results were compared with those in untreated GK rats, and wild-type animals. Results: Micro-computed tomography, histomorphometry, and bone mineral density analysis demonstrated that T2DM induced significant osteopenia, and impairment of bone microarchitecture and biomechanical properties in GK rats. T2DM also significantly decreased bone formation and increased bone resorption parameters in three regions of the skeleton (proximal tibia, mid-shaft of the tibia, and lumbar vertebrae), and increased carboxy-terminal type I collagen crosslinks, tartrate-resistant acid phosphatase, muscle ubiquitin C, and bone thioredoxin interacting protein (TXNIP) expression. Calcitriol treatment significantly alleviated bone loss, and improved bone microarchitecture and biomechanical properties and also decreased serum glucose and glycated serum protein levels. Biomarkers of bone formation were significantly increased, while muscle ubiquitin C and bone TXNIP expression were significantly decreased following calcitriol treatment. Conclusions:These results suggest that 1,25-dihydroxy vitamin D3 treatment effectively attenuates osteopenia, and improves bone and muscle quality in GK type 2 diabetes model rats.

引用

页码：184 / 195

页数：12

共 33 条

[1] Low 25-Hydroxyvitamin D and Risk of Type 2 Diabetes: A Prospective Cohort Study and Metaanalysis [J].

Afzal, Shoaib ;

Bojesen, Stig E. ;

Nordestgaard, Borge G. .

CLINICAL CHEMISTRY, 2013, 59 (02) :381-391

[2] Skeletal changes in type-2 diabetic Goto-Kakizaki rats [J].

Ahmad, T ;

Ohlsson, C ;

Sääf, M ;

Östenson, CG ;

Kreicbergs, A .

JOURNAL OF ENDOCRINOLOGY, 2003, 178 (01) :111-116

[3] Role of Vitamin D in Insulin Secretion and Insulin Sensitivity for Glucose Homeostasis [J].

Alvarez, Jessica A. ;

Ashraf, Ambika .

INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, 2010, 2010

[4] RETRACTED: Effects of vitamin D supplementation on glucose metabolism, lipid concentrations, inflammation, and oxidative stress in gestational diabetes: a double-blind randomized controlled clinical trial (Publication with Expression of Concern. See vol. 112, pg. 1406, 2020) (Retracted article. See vol. 113, pg. 1382, 2021) [J].

Asemi, Zatollah ;

Hashemi, Teibeh ;

Karamali, Maryam ;

Samimi, Mansooreh ;

Esmaillzadeh, Ahmad .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 2013, 98 (06) :1425-1432

[5] Vitamin D metabolism, functions and needs: from science to health claims [J].

Battault, S. ;

Whiting, S. J. ;

Peltier, S. L. ;

Sadrin, S. ;

Gerber, G. ;

Maixent, J. M. .

EUROPEAN JOURNAL OF NUTRITION, 2013, 52 (02) :429-441

[6]

Borissova AM, 2003, INT J CLIN PRACT, V57, P258

[7] Reactive oxygen species and oxidative stress in osteoclastogenesis, skeletal aging and bone diseases [J].

Callaway, Danielle A. ;

Jiang, Jean X. .

JOURNAL OF BONE AND MINERAL METABOLISM, 2015, 33 (04) :359-370

[8] VITAMIN-D(3) AND CALCIUM TO PREVENT HIP-FRACTURES IN ELDERLY WOMEN [J].

CHAPUY, MC ;

ARLOT, ME ;

DUBOEUF, F ;

BRUN, J ;

CROUZET, B ;

ARNAUD, S ;

DELMAS, PD ;

MEUNIER, PJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (23) :1637-1642

[9] Standardized Nomenclature, Symbols, and Units for Bone Histomorphometry: A 2012 Update of the Report of the ASBMR Histomorphometry Nomenclature Committee [J].

Dempster, David W. ;

Compston, Juliet E. ;

Drezner, Marc K. ;

Glorieux, Francis H. ;

Kanis, John A. ;

Malluche, Hartmut ;

Meunier, Pierre J. ;

Ott, Susan M. ;

Recker, Robert R. ;

Parfitt, A. Michael .

JOURNAL OF BONE AND MINERAL RESEARCH, 2013, 28 (01) :1-16

[10] Oral vitamin D3 and calcium for secondary prevention of low-trauma fractures in elderly people (Randomised Evaluation of Calcium Or vitamin D, RECORD): a randomised placebo-controlled trial [J].

Grant, AM ;

Anderson, FH ;

Avenell, A ;

Campbell, MK ;

Cooper, C ;

Donaldson, C ;

Francis, RM ;

Gillespie, WJ ;

Robinson, CM ;

Torgerson, DJ ;

Wallace, WA ;

McPherson, GC ;

MacLennan, GS ;

McDonald, AM ;

Grant, M ;