A comparison of faecal examination, commercial ELISA kit, and indirect-ELISA methods in the diagnosis of sheep fasciolosis

被引：5

作者：

Utuk, Armagan Erdem ^{[2
]}

Simsek, Sami ^{[1
]}

Koroglu, Ergun ^{[1
]}

机构：

[1] Firat Univ, Dept Parasitol, Fac Vet Med, TR-23119 Elazig, Turkey

[2] Etlik Vet Control Cent Res Inst, Parasitol & Bee Dis Lab, TR-06020 Ankara, Turkey

来源：

SMALL RUMINANT RESEARCH | 2012年 / 107卷 / 2-3期

关键词：

Fasciola hepatica; Commercial ELISA kit; Home-made indirect ELISA; Sheep; LINKED-IMMUNOSORBENT-ASSAY; HEPATICA INFECTION; NW SPAIN; CATTLE; GALICIA;

D O I：

10.1016/j.smallrumres.2012.05.006

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of this study was to compare the diagnosis of sheep fasciolosis by faecal examination, commercial ELISA kit, and home-made indirect ELISA method. For this purpose, a total of 265 sheep faecal samples were examined with the sedimentation method and 185 sheep serum samples were examined both with a commercial ELISA kit and a home-made indirect ELISA method. The prevalence of Fasciola hepatica eggs was 4.9%. The prevalence of anti-F. hepatica antibodies was 16.2% using the commercial ELISA kit and 15.6% using the home-made indirect ELISA method. It is concluded that although the faecal examination is the "gold standard" in the diagnosis of sheep fasciolosis, serological tests should not be ruled out, and particularly the home-made indirect ELISA method may be preferred, since it is less expensive than commercial kits and has similar reliability rates. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：164 / 166

页数：3