Biohydrogen production from palm oil mill effluent using immobilized Clostridium butyricum EB6 in polyethylene glycol

被引:39
作者
Singh, Lakhveer [1 ]
Wahid, Zularisam A. [2 ]
Siddiqui, Muhammad Faisal [3 ]
Ahmad, Anwar [4 ]
Ab Rahim, Mohd Hasbi [1 ]
Sakinah, Mimi [3 ]
机构
[1] Univ Malaysia Pahang, Fac Ind Sci & Technol, Kuantan 26300, Pahang, Malaysia
[2] Univ Malaysia Pahang, Fac Civil Engn & Earth Resources, Kuantan 26300, Pahang, Malaysia
[3] Univ Malaysia Pahang, Fac Chem & Nat Resource Engn, Kuantan 26300, Pahang, Malaysia
[4] King Saud Univ KSA, Coll Engn, Dept Civil Engn, Riyadh 11421, Saudi Arabia
关键词
Biohydrogen; Clostridium butyricum EB6; Palm oil mill effluent (POME); Polyethylene glycol; Immobilization; BIO-HYDROGEN PRODUCTION; RETENTION TIME; SEWAGE-SLUDGE; FOOD WASTE; FERMENTATION; OPTIMIZATION;
D O I
10.1016/j.procbio.2012.12.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium butyricum EB6 to improve biohydrogen (bio-H-2) production from palm oil mill effluent (POME). POME is used as a substrate that can act as a carbon source. The resulting PEG-immobilized cells were found to yield 5.35 LH2/L-POME, and the maximum H-2 production rate was 510 mL H-2/L-POME h (22.7 mmol/L h). The Monod-type kinetic model was used to describe the effect of substrate (POME) concentration on the H-2 production rate. The acclimation of immobilized cells greatly improved H-2 production. Batch experiments demonstrated that particle size of PEG-immobilized cells for efficient H-2 production 3 mm. It is significant that this is the first report on whole-cell immobilization in PEG for H-2 production from POME. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:294 / 298
页数:5
相关论文
共 25 条
[1]  
[Anonymous], 1998, Standard methods of examination of water and waste water, P1220
[2]   Fed batch production of hydrogen from palm oil mill effluent using anaerobic microflora [J].
Atif, AAY ;
Fakhru'l-Razi, A ;
Ngan, MA ;
Morimoto, M ;
Iyuke, SE ;
Veziroglu, NT .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2005, 30 (13-14) :1393-1397
[3]   Effect of hydraulic retention time on biohydrogen production from palm oil mill effluent in anaerobic sequencing batch reactor [J].
Badiei, Marzieh ;
Jahim, Jamaliah Md ;
Anuar, Nurina ;
Rozaimah, Siti ;
Abdullah, Sheikh .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2011, 36 (10) :5912-5919
[4]   Enhanced biohydrogen production from sewage sludge with alkaline pretreatment [J].
Cai, ML ;
Liu, JX ;
Wei, YS .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2004, 38 (11) :3195-3202
[5]   Biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent [J].
Chong, Mei-Ling ;
Rahim, Raha Abdul ;
Shirai, Yoshihito ;
Hassan, Mohd Ali .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2009, 34 (02) :764-771
[6]   IMMOBILIZED MICROORGANISMS FOR HYDROGEN AND AMMONIA PRODUCTION [J].
HALLENBECK, PC .
ENZYME AND MICROBIAL TECHNOLOGY, 1983, 5 (03) :171-180
[7]   Pretreatment of methanogenic granules for immobilized hydrogen fermentation [J].
Hu, Bo ;
Chen, Shulin .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2007, 32 (15) :3266-3273
[8]   Continuous fermentative hydrogen production from coffee drink manufacturing wastewater by applying UASB reactor [J].
Jung, Kyung-Won ;
Kim, Dong-Hoon ;
Shin, Hang-Sik .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2010, 35 (24) :13370-13378
[9]   THE IMMOBILIZATION OF WHOLE CELLS - ENGINEERING PRINCIPLES [J].
KAREL, SF ;
LIBICKI, SB ;
ROBERTSON, CR .
CHEMICAL ENGINEERING SCIENCE, 1985, 40 (08) :1321-1354
[10]   Comparative analysis of thermophilic immobilized biohydrogen production using packed materials of ceramic ring and pumice stone [J].
Keskin, Tugba ;
Aksoyek, Elif ;
Azbar, Nuri .
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, 2011, 36 (23) :15160-15167