In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins

被引：17
作者：
Sciolino, Nicholas [1 ]
Burz, David S. [1 ]
Shekhtman, Alexander [1 ]
机构：
[1] SUNY Albany, Dept Chem, Albany, NY 12222 USA
来源：
PROTEOMICS | 2019年 / 19卷 / 06期
关键词：
in-cell NMR; Mycobacterium tuberculosis; NMR spectroscopy; protein-protein interactions; synuclein; UBIQUITIN-LIKE PROTEIN; HUMAN ALPHA-SYNUCLEIN; A-BETA COMPONENT; MYCOBACTERIUM-TUBERCULOSIS; ESCHERICHIA-COLI; ALZHEIMERS-DISEASE; QUINARY INTERACTIONS; SECONDARY STRUCTURE; BACKBONE DYNAMICS; HYDROGEN-EXCHANGE;
D O I：
10.1002/pmic.201800055
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This review summarizes the results of in-cell Nuclear Magnetic Resonance, NMR, spectroscopic investigations of the eukaryotic and prokaryotic intrinsically disordered proteins, IDPs: alpha-synuclein, prokaryotic ubiquitin-like protein, Pup, tubulin-related neuronal protein, Tau, phenylalanyl-glycyl-repeat-rich nucleoporins, FG Nups, and the negative regulator of flagellin synthesis, FlgM. The results show that the cellular behavior of IDPs may differ significantly from that observed in the test tube.
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页数：16
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