Identification of human rotavirus strains with the P[14] genotype by PCR

被引：28
作者：
Arista, S
Vizzi, E
Alaimo, C
Palermo, D
Cascio, A
机构：
[1] Univ Palermo, Dipartimento Igine & Microbiol, I-90127 Palermo, Italy
[2] Univ Palermo, Osped Bambini G Di Cristina, Ist Patol Infettiva & Virol, Palermo, Italy
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 08期
关键词：
D O I：
10.1128/JCM.37.8.2706-2708.1999
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
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页码：2706 / 2708
页数：3
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