Topical Application of Propolis Enhances Cutaneous Wound Healing by Promoting TGF-Beta/Smad-Mediated Collagen Production in a Streptozotocin-Induced Type I Diabetic Mouse Model

被引：106

作者：

Hozzein, Wael N. ^{[1
,2
]}

Badr, Gamal ^{[3
]}

Al Ghamdi, Ahmad A. ^{[4
]}

Sayed, Ayat ^{[5
]}

Al-Waili, Noori S. ^{[6
]}

Garraud, Olivier ^{[7
,8
]}

机构：

[1] King Saud Univ, Coll Sci, Dept Zool, Bioprod Res Chair, Riyadh 11451, Saudi Arabia

[2] Beni Suef Univ, Fac Sci, Bot Dept, Bani Suwayf, Egypt

[3] Assiut Univ, Fac Sci, Dept Zool, Lab Immunol & Mol Physiol, Assiut 71516, Egypt

[4] King Saud Univ, Coll Food & Agr Sci, Chair Engineer Abdullah Baqshan Bee Res, Riyadh, Saudi Arabia

[5] Assiut Univ, Fac Med, Dept Biochem, Assiut 71516, Egypt

[6] Al Wailis Fdn Sci, New York, NY USA

[7] Inst Natl Transfus Sanguine, F-75015 Paris, France

[8] Univ Lyon, St Etienne, France

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 37卷 / 03期

关键词：

Cytokines; Diabetes mellitus; Inflammation; Propolis; Wound healing; UNDENATURED WHEY-PROTEIN; GROWTH-FACTOR-BETA; TISSUE INHIBITOR; HUMAN SKIN; EXPRESSION; MICE; FOOT; MATRIX-METALLOPROTEINASE-9; IMPROVES; REPAIR;

D O I：

10.1159/000430221

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Impaired wound healing is considered to be one of the most serious complications associated with diabetes as it significantly increases the susceptibility of patients to infection. Propolis is a natural bee product used extensively in foods and beverages that has significant benefits to human health. In particular, propolis has antioxidant, anti-inflammatory and analgesic effects that could be useful for improving wound healing. In this study, we investigated the effects of topical application of propolis on the healing and closure of diabetic wounds in a streptozotocin (STZ)-induced type I diabetic mouse model. Methods: Sixty male mice were distributed equally into 3 experimental groups: group 1, non-diabetic control mice; group 2, diabetic mice; and group 3, diabetic mice treated daily with a topical application of propolis. Results: We found that diabetic mice exhibited delayed wound closure characterized by a significant decrease in the levels of TGF-beta 1 and a prolonged elevation of the levels of inflammatory cytokines (IL-beta, IL-6 and INF-alpha) and MMP9 in wound tissues compared with control non-diabetic mice. Moreover, the wound tissues of diabetic mice showed a marked reduction in the phosphorylation of Smad2 and Smad3 as well as a marked reduction in collagen production. Interestingly, compared with untreated diabetic mice, topical application of propolis significantly enhanced the closure of diabetic wounds and decreased the levels of IL-1 beta, IL-6, INF-alpha and MMP9 to near normal levels. Most importantly, compared with untreated diabetic mice, the treatment of diabetic mice with propolis significantly enhanced the production of collagen via the TGF-beta 1/Smad2,3 signaling axis in wounded tissues. Conclusion: Our findings reveal the molecular mechanisms underlying the improved healing and closure of diabetic wounds following topical propolis application. Copyright (C) 2015 S. Karger AG, Basel

引用

页码：940 / 954

页数：15

共 60 条

[1] Tumor necrosis factor-α-accelerated degradation of type I collagen in human Skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo [J].