Effect of extremely low frequency magnetic fields on calpain activation

被引：12

作者：

Salamino, F

Minafra, R

Grano, V

Diano, N

Mita, DG

Pontremoli, S

Melloni, E

机构：

[1] CNR, Inst Genet & Biophys A Buzzati Traverso, I-80125 Naples, Italy

[2] Univ Genoa, Dept Expt Med, Biochem Sect, Genoa, Italy

[3] Univ Genoa, Ctr Excellence Biomed Res, Genoa, Italy

[4] Univ Naples 2, Dept Expt Med, Naples, Italy

来源：

BIOELECTROMAGNETICS | 2006年 / 27卷 / 01期

关键词：

ELF magnetic fields; calcium ions; enzyme activation; proteolysis;

D O I：

10.1002/bem.20176

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of low intensity, low frequency magnetic fields (MFs) on catalytic activity of the calcium dependent protease calpain was determined following the enzyme activation both in "in vitro" and "in vivo" conditions. We have observed that a 0.3 mT MF induces a significant increase in the requirement of the protease for this metal ion. This change is detectable at low [Ca2+] and disappears when the level of Ca2+ is raised to saturating amounts. The observed effects are not due to transient MF- induced conformational changes occurring in calpain, but to direct effects of the MF on Ca2+ ions, which become less available for the binding sites present in calpain. Altogether, these results indicate that exposure to low intensity, low frequency MFs alters the intracellular Ca2+, availability," thereby modifying the related cell response.

引用

页码：43 / 50

页数：8