Alternatively SplicedBnaPAP2.A7Isoforms Play Opposing Roles in Anthocyanin Biosynthesis ofBrassica napusL.

被引：32

作者：

Chen, Daozong ^{[1
]}

Liu, Yi ^{[1
]}

Yin, Shuai ^{[1
]}

Qiu, Jie ^{[1
]}

Jin, Qingdong ^{[1
]}

King, Graham J. ^{[2
]}

Wang, Jing ^{[1
]}

Ge, Xianhong ^{[1
]}

Li, Zaiyun ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Ctr Oil Crop Improvement Wuhan, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

[2] Southern Cross Univ, Southern Cross Plant Sci, Lismore, NSW, Australia

来源：

FRONTIERS IN PLANT SCIENCE | 2020年 / 11卷

关键词：

Brassica napusL; anthocyanin; BnaPAP2; A7; alternative splicing; MYB TRANSCRIPTION FACTORS; FLAVONOID BIOSYNTHESIS; REGULATORY NETWORK; MOLECULAR ANALYSIS; DNA-BINDING; ARABIDOPSIS; ACCUMULATION; PROTEIN; GENES; EVOLUTIONARY;

D O I：

10.3389/fpls.2020.00983

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Brassica napusL. (rapeseed, oilseed rape, and canola) and varieties of its two diploid parents,B. oleraceaandB. rapa, display a large amount of variation in anthocyanin pigmentation of the leaf, stem, and fruit. Here, we demonstrate thatBnaPAP2.A7, an ortholog of theB. oleraceaanthocyanin activatorBoMYB2that confers purple traits, positively regulates anthocyanin biosynthesis in leaves ofB. napus. Sequencing ofBnaPAP2.A7and transgenic analysis suggests that activation of this gene in purple rapeseed may result from a single nucleotide and/or 2bp insertion in its promoter region.BnaPAP2.A7gives rise to three splice variants, designatedBnaPAP2.A7-744, BnaPAP2.A7-910, andBnaPAP2.A7-395according to the length of the transcripts. WhileBnaPAP2.A7-744encodes a full-length R2R3-MYB, bothBnaPAP2.A7-910andBnaPAP2.A7-395encode truncated proteins that lack both a partial R3 repeat and the complete C terminal domain, and soin vitroare unable to interact with theArabidopsisbHLH protein AtTT8. Although expression of eitherBnaPAP2.A7-910orBnaPAP2.A7-395in green rapeseed does not result in purple leaves, both genes do modify genome-wide gene expression, with a strong repression of anthocyanin-related genes. We have demonstrated thatBnaPAP.A7regulates anthocyanin accumulation in leaves ofB. napusand propose a potential mechanism for modulation of anthocyanin biosynthesis by alternative splicing.

引用

页数：14

共 70 条

[1] The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco [J].