Whole genome amplification of the obligate intracellular pathogen Coxiella burnetii using multiple displacement amplification

被引：3

作者：

Kumar, Sanjay ^{[1
]}

Gangoliya, Shefali Raj ^{[1
]}

Berri, Mustapha ^{[2
]}

Rodolakis, Annie ^{[2
]}

Alam, Syed Imteyaz ^{[1
]}

机构：

[1] Def Res & Dev Estab, Div Biotechnol, Gwalior 474002, India

[2] INRA, ISP UMR1282, F-37380 Nouzilly, France

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 95卷 / 03期

关键词：

Coxiella burnetii; Whole genome amplification; Multiple displacement amplification; DNA-POLYMERASE;

D O I：

10.1016/j.mimet.2013.10.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study demonstrates that whole genome multiple displacement amplification (MDA) is a promising technique for downstream genomic analysis of fastidious obligate intracellular pathogens such as Coxiella burnetii. The MDA technology can help in obtaining sufficient genetic material from highly infectious agent and thus minimizing repeated culturing and associated biohazard. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：368 / 372

页数：5

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