CRISPR/Cas9 in zebrafish: an efficient combination for human genetic diseases modeling

被引：61

作者：

Liu, Jiaqi ^{[1
,2
,3
,4
,5
]}

Zhou, Yangzhong ^{[3
,6
,7
]}

Qi, Xiaolong ^{[8
]}

Chen, Jia ^{[1
,2
,3
,6
]}

Chen, Weisheng ^{[1
,2
,3
,6
]}

Qiu, Guixing ^{[1
,2
,3
,6
]}

Wu, Zhihong ^{[2
,3
,6
,9
]}

Wu, Nan ^{[1
,2
,3
,6
]}

机构：

[1] Beijing Union Med Coll Hosp, Peking Union Med Coll, Dept Orthopaed Surg, 1 Shuaifuyuan, Beijing 100730, Peoples R China

[2] Chinese Acad Med Sci, 1 Shuaifuyuan, Beijing 100730, Peoples R China

[3] Beijing Key Lab Genet Res Skeletal Deform, Beijing, Peoples R China

[4] Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Dept Breast Surg Oncol, Beijing, Peoples R China

[5] Peking Union Med Coll, Beijing, Peoples R China

[6] Chinese Acad Med Sci, Med Res Ctr Orthopaed, Beijing, Peoples R China

[7] Tsinghua Univ, Sch Med, Beijing, Peoples R China

[8] Southern Med Univ, Nanfang Hosp, Dept Gen Surg, Guangzhou, Guangdong, Peoples R China

[9] Beijing Union Med Coll Hosp, Peking Union Med Coll, Dept Cent Lab, 1 Shuaifuyuan, Beijing 100730, Peoples R China

来源：

HUMAN GENETICS | 2017年 / 136卷 / 01期

基金：

中央高校基本科研业务费专项资金资助; 中国国家自然科学基金;

关键词：

HUMAN GENOME; SEQUENCE VARIANTS; DNA CLEAVAGE; DANIO-RERIO; CAS SYSTEM; TARGET DNA; GUIDE RNA; IDENTIFICATION; MUTAGENESIS; PROKARYOTES;

D O I：

10.1007/s00439-016-1739-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The next-generation sequencing identifies a growing number of candidate genes associated with human genetic diseases, which inevitably requires efficient methods to validate the causal links between genotype and phenotype. Recently, zebrafish, with sufficiently high-throughput capabilities, has become a favored option to study human pathogenesis. In addition, CRISPR/Cas9-based approaches have radically reduced the efforts to introduce targeted genome engineering in various organisms. Here, we systemically review the basic considerations in the design of gene editing in zebrafish with CRISPR/Cas9, and explore the potential of the combination of these two to support efficient functional analysis of human genetic variants.

引用

页码：1 / 12

页数：12

共 97 条

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