Effects of some drugs on human cord blood erythrocyte carbonic anhydrases I and II: an in vitro study

被引：8

作者：

Cankaya, Murat ^{[2
]}

Aktas, Mehmet ^{[1
]}

Kuzucu, Mehmet ^{[2
]}

Gul, Ilhami ^{[3
]}

Coban, T. Abdulkadir ^{[1
]}

机构：

[1] Erzincan Univ, Fac Med, Dept Biochem, Erzincan, Turkey

[2] Erzincan Univ, Sci & Art Fac, Dept Biol, Erzincan, Turkey

[3] Erzincan Mil Hosp, Womens Dis & Matern Ward, Erzincan, Turkey

来源：

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY | 2012年 / 27卷 / 05期

关键词：

Carbonic anhydrase; cord blood; erythrocytes; ESTERASE-ACTIVITIES; ISOZYMES; INHIBITION; ALPHA; BETA;

D O I：

10.3109/14756366.2011.604852

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, we purified hcbCA I and II from human cord blood erythrocytes using by Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Also, it was checked the inhibition effects of ampicillin sulfate, ceftriaxone, ceftizoxime and ranitidine on hcbCA I and hcbCA II. IC50 values for ceftriaxone, ceftizoxime and ranitidine were found to be 27.l, 79.4 and 55.5 mu M for hcbCA I and of 21.0, 79.1 and 66.1 mu M for hcbCA II, respectively. According to these results, Ampicillin sulfate inhibited only hcbCAII and IC50 values of this antibiotic was found to be 56.8 mu M. All these substances were found non-competitive inhibitors. It is important to study the inhibition effects of these drugs on hcbCA I and II izoenzymes. Because, pregnant woman is take all of these substance. For this reason, these drugs should be carefully used and the dosage should be very well ordered to minimize side effects.

引用

页码：641 / 645

页数：5

共 36 条

[1]

[Anonymous], 2006, AHFS DRUG INFORM

[2] HIGH-LEVELS OF INTERLEUKIN-10 PRODUCTION IN-VIVO ARE ASSOCIATED WITH TOLERANCE IN SCID PATIENTS TRANSPLANTED WITH HLA MISMATCHED HEMATOPOIETIC STEM-CELLS [J].

BACCHETTA, R ;

BIGLER, M ;

TOURAINE, JL ;

PARKMAN, R ;

TOVO, PA ;

ABRAMS, J ;

MALEFYT, RD ;

DEVRIES, JE ;

RONCAROLO, MG .

JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (02) :493-502

[3] Synthesis and carbonic anhydrase inhibitory properties of novel bromophenols including natural products [J].

Balaydin, Halis Turker ;

Soyut, Hakan ;

Ekinci, Deniz ;

Goksu, Suleyman ;

Beydemir, Sukru ;

Menzek, Abdullah ;

Sahin, Ertan .

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 2012, 27 (01) :43-50

[4]

Barclay L, 2007, MMWR-MORBID MORTAL W, V56, P332

[5] Effects of some medical drugs on enzyme activities of carbonic anhydrase from human erythrocytes in vitro and from rat erythrocytes in vivo [J].

Beydemir, S ;

Çiftçi, M ;

Özmen, I ;

Buyukokuroglu, ME ;

Özdemir, H ;

Küfrevioglu, ÖI .

PHARMACOLOGICAL RESEARCH, 2000, 42 (02) :187-191

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7]

Brinkman RMR., 1932, Journal of Physiology, V75, P3

[8]

Britta K., 1997, J PLANT PHYSL, V150, P137

[9] Placental and/or umbilical cord blood: An alternative source of hematopoietic stem cells for transplantation [J].

Cairo, MS ;

Wagner, JE .

BLOOD, 1997, 90 (12) :4665-4678

[10] Purification and characterization of carbonic anhydrase from the teleost fish Dicentrarchus labrax (European seabass) liver and toxicological effects of metals on enzyme activity [J].

Ceyhun, Saltuk Bugrahan ;

Senturk, Murat ;

Yerlikaya, Emrah ;

Erdogan, Orhan ;

Kufrevioglu, Omer Irfan ;

Ekinci, Deniz .

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2011, 32 (01) :69-74

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