Application of Hydrophilic Interaction Chromatography in Phytochemical Analysis

被引:11
作者
Petruczynik, A. [1 ]
Waksmundzka-Hajnos, M. [1 ]
机构
[1] Med Univ Lublin, Dept Inorgan Chem, PL-20093 Lublin, Poland
关键词
HILIC; hydrophilic interaction chromatography; phytochemical analysis; carbohydrates; amino acids; peptides; phenolic acids; flavonoids; alkaloids; PERFORMANCE LIQUID-CHROMATOGRAPHY; SILICA STATIONARY-PHASE; REVERSED-PHASE; RETENTION MECHANISM; CAPILLARY COLUMNS; POLAR COMPOUNDS; SEPARATION; PEPTIDES; HPLC; SELECTIVITY;
D O I
10.1556/AChrom.25.2013.1.1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Hydrophilic interaction chromatography (HILIC) is a liquid chromatography technique when a polar stationary phases - silica or polar bonded phases and aqueous mobile phase containing amount of a less polar solvent (often acetonitrile) is applied. HILIC is important for the separation of highly polar substances including biologically active compounds, such as drugs, amino acids, peptides, proteins, nucleosides, neurotransmitters, etc. HILIC is also the appropriate method for analysis of plant extract polar components such as carbohydrates, amino acids, peptides, phenolic acids, flavonoids and some alkaloids. Plant extracts are usually multicomponent mixtures of compounds of wide polarity range, which often cannot be analyzed by use of a single separation and detection method because of the high chemical diversity. Good results are obtained by use of coupled method, e.g., reversed-phase liquid chromatography (RP-LC) and HILIC mode separation. The elution order in HILIC is usually opposite to that in RP separations. This orthogonality determines one of the advantages of HILIC - the possibility of creating multidimensional separations. An important feature of HILIC is the improved sensitivity with electrospray mass spectrometry. This is significant for the analysis of components existing in small concentration in multicomponent mixtures. The high acetonitrile content also gives additional advantage of HILIC - faster separations due to the lower viscosity of HILIC eluents compared to standard RP ones. The presented review deals with the optimization of HILIC separations and application of the method for analysis of plant extracts components.
引用
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页码:1 / 25
页数:25
相关论文
共 65 条
[1]  
Albersheim P., 1967, CARBOHYD RES, V5, P3430
[2]   HYDROPHILIC-INTERACTION CHROMATOGRAPHY OF COMPLEX CARBOHYDRATES [J].
ALPERT, AJ ;
SHUKLA, M ;
SHUKLA, AK ;
ZIESKE, LR ;
YUEN, SW ;
FERGUSON, MAJ ;
MEHLERT, A ;
PAULY, M ;
ORLANDO, R .
JOURNAL OF CHROMATOGRAPHY A, 1994, 676 (01) :191-202
[3]   HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].
ALPERT, AJ .
JOURNAL OF CHROMATOGRAPHY, 1990, 499 :177-196
[4]   Hydrophilic interaction chromatography/electrospray mass spectrometry analysis of carbohydrate-related metabolites from Arabidopsis thaliana leaf tissue [J].
Antonio, Carla ;
Larson, Tony ;
Gilday, Alison ;
Graham, Ian ;
Bergstroem, Ed ;
Thomas-Oates, Jane .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2008, 22 (09) :1399-1407
[5]   Retention pattern profiling of fungal metabolites on mixed-mode reversed-phase/weak anion exchange stationary phases in comparison to reversed-phase and weak anion exchange separation materials by liquid chromatography-electrospray ionisation-tandem mass spectrometry [J].
Apfelthaler, Elisabeth ;
Bicker, Wolfgang ;
Laemmerhofer, Michael ;
Sulyok, Michael ;
Krska, Rudolf ;
Lindner, Wolfgang ;
Schuhmacher, Rainer .
JOURNAL OF CHROMATOGRAPHY A, 2008, 1191 (1-2) :171-181
[6]   Practice and mechanism of HPLC oligosaccharide separation with a cyclodextrin bonded phase [J].
Berthod, A ;
Chang, SSC ;
Kullman, JPS ;
Armstrong, DW .
TALANTA, 1998, 47 (04) :1001-1012
[7]   Separation of high molecular weight saponins of Archidendron ellipticum by hydrophilic interaction chromatography [J].
Beutler, JA .
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1997, 20 (15) :2415-2426
[8]   HPLC columns partition by chemometric methods based on peptides retention [J].
Buszewski, Boguslaw ;
Kowalska, Sylwia ;
Kowalkowski, Tomasz ;
Rozpedowska, Katarzyna ;
Michel, Monika ;
Jonsson, Tobias .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 845 (02) :253-260
[9]   Microwave-assisted chemistry of carbohydrates [J].
Corsaro, A ;
Chiacchio, U ;
Pistarà, V ;
Romeo, G .
CURRENT ORGANIC CHEMISTRY, 2004, 8 (06) :511-538
[10]   New insights into the regulation of phytochelatin biosynthesis in A-thaliana cells from metabolite profiling analyses [J].
Ducruix, C. ;
Junot, C. ;
Fievet, J. -B. ;
Villiers, F. ;
Ezan, E. ;
Bourguignon, J. .
BIOCHIMIE, 2006, 88 (11) :1733-1742