Quantal acetylcholine release induced by mediatophore transfection

被引：56

作者：

FalkVairant, J

Correges, P

EderColli, L

Salem, N

Roulet, E

Bloc, A

Meunier, F

Lesbats, B

Loctin, F

Synguelakis, M

Israel, M

Dunant, Y

机构：

[1] CNRS,NEUROBIOL CELLULAIRE & MOLEC LAB,F-91198 GIF SUR YVETTE,FRANCE

[2] CNRS,INST ALFRED FESSARD,F-91198 GIF SUR YVETTE,FRANCE

[3] CTR MED UNIV GENEVA,DEPT PHARMACOL,CH-1211 GENEVA,SWITZERLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 11期

关键词：

neurotransmitter release; neuroblastoma cells; Xenopus myocytes;

D O I：

10.1073/pnas.93.11.5203

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mediatophore is a protein of approximate to 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed insitu in cholinergic synapses from which mediatophore was purified.

引用

页码：5203 / 5207

页数：5

共 26 条

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