Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT

被引：46
作者：
Xu, Xiaojun [1 ]
Liu, Xiaoyuan [2 ]
Zhang, Yan [3 ]
机构：
[1] Second Peoples Hosp Wuxi, Dept Pharm, Wuxi, Peoples R China
[2] Forth Peoples Hosp Wuxi, Dept Pharm, Wuxi, Peoples R China
[3] Wuxi M & Child Hlth Hosp, Dept Pharm, Wuxi, Peoples R China
来源：
PLOS ONE | 2018年 / 13卷 / 03期
基金：
中国国家自然科学基金;
关键词：
CYCLE ARREST; HEPATOCELLULAR-CARCINOMA; SIGNALING PATHWAY; A549; CELLS; APOPTOSIS; ACTIVATION;
D O I：
10.1371/journal.pone.0193449
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Osthole is an active compound isolated from Chinese herb Cnidium monnieri (L.) Cusson, and had been reported to possess antitumor effect. However, the effect of osthole on the gastric cancer cells has not been investigated. In this study, the effects of osthole on the proliferation of human gastric cancer cells were tested. The data showed that osthole treatment significantly inhibited the proliferation of gastric cancer cells and resulted in the cell cycle arrest at G2/M phase in a dose-dependent manner. Western-blot study showed that the expression of cyclin B1 and cdc2 was markedly reduced by osthole. Moreover, expression of P13K and pAKT was also significantly suppressed, and the results indicated that the inhibition of pAKT, cyclin B1, and cdc2 levels by osthole was notably enhanced by a P13K inhibitor. These results demonstrate that osthole could inhibit gastric cancer cells proliferation via induction of cell cycle arrest at G2/M phase by the reduction of PI3K/AKT.
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页数：10
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