Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor

被引：39

作者：

Sakamoto, Akihiko ^{[1
]}

Terui, Yusuke ^{[1
]}

Yamamoto, Taku ^{[1
]}

Kasahara, Takuma ^{[1
]}

Nakamura, Mizuho ^{[1
]}

Tomitori, Hideyuki ^{[1
]}

Yamamoto, Kaneyoshi ^{[2
]}

Ishihama, Akira ^{[2
]}

Michael, Anthony J. ^{[3
]}

Igarashi, Kazuei ^{[4
,5
]}

Kashiwagi, Keiko ^{[1
]}

机构：

[1] Chiba Inst Sci, Fac Pharm, Choshi, Chiba 2880025, Japan

[2] Hosei Univ, Dept Frontier Biosci, Koganei, Tokyo 1848584, Japan

[3] Univ Texas SW Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA

[4] Chiba Univ, Grad Sch Pharmaceut Sci, Chuo Ku, Chiba 2608675, Japan

[5] Chiba Univ, Amine Pharma Res Inst, Chuo Ku, Chiba 2600856, Japan

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | 2012年 / 44卷 / 11期

关键词：

Polyamine modulon; Biofilm formation; Cell viability; Two-component signal transducing system; Ribosome recycling factor; ESCHERICHIA-COLI; RNA-POLYMERASE; MODULATION FACTOR; GROWTH-PHASE; PROTEIN; EXPRESSION; ACTIVATION; MUTANTS; ABSENCE; SUBUNIT;

D O I：

10.1016/j.biocel.2012.07.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have reported that polyamines increase cell viability at the stationary phase of cell growth through translational stimulation of ribosome modulation factor, and SpoT and RpoZ proteins involved in the synthesis and function of ppGpp in Escherichia coli. Since biofilm formation is also involved in cell viability, we looked for proteins involved in biofilm formation and cell viability whose synthesis is stimulated by polyamines at the level of translation. It was found that the synthesis of response regulators UvrY and CpxR in the two-component signal transducing systems and ribosome recycling factor (RRF) was increased by polyamines at the level of translation. Polyamine stimulation of the synthesis of UvrY and RRF was dependent on the existence of the inefficient initiation codons UUG and GUG in uvrY and frr mRNA, respectively; and polyamine stimulation of CpxR synthesis was dependent on the existence of an unusual location of a Shine-Dalgarno (SD) sequence in cpxR mRNA. Biofilm formation and cell viability in the absence of polyamines was increased by transformation of modified uvrY and cpxR genes, and cell viability by modified frr gene whose translation occurs effectively without polyamines. The results indicate that polyamines are necessary for both biofilm formation and cell viability. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1877 / 1886

页数：10

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